Quality of histone modification antibodies undermines chromatin biology research.
Quality of histone modification antibodies undermines chromatin biology research.
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DOI：
10.12688/f1000research.7265.2
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发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Jeltsch, Albert
中科院分区：
文献类型：
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作者：
Kungulovski, Goran;Jeltsch, Albert
组蛋白翻译后修饰（PTM）抗体是染色质生物学中必不可少的研究试剂。然而，它们的性能不稳定，质量证明文件不足。抗体制造商和供应商应提供详细的批次特定质量文件，使最终客户无需进行进一步的质量检查。从多克隆抗体向可持续试剂如单克隆或重组抗体或组蛋白结合结构域的转变将有助于提高该领域实验工作的再现性。
Histone post-translational modification (PTM) antibodies are essential research reagents in chromatin biology. However, they suffer from variable properties and insufficient documentation of quality. Antibody manufacturers and vendors should provide detailed lot-specific documentation of quality, rendering further quality checks by end-customers unnecessary. A shift from polyclonal antibodies towards sustainable reagents like monoclonal or recombinant antibodies or histone binding domains would help to improve the reproducibility of experimental work in this field.