Effects of breathing and cardiac motion on spatial resolution in the microscopic imaging of rodents
Effects of breathing and cardiac motion on spatial resolution in the microscopic imaging of rodents
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DOI：
10.1002/mrm.20400
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发表时间：
2005-04-01
影响因子：
3.3
通讯作者：
Johnson, GA
中科院分区：
文献类型：
--
作者：
Maï, W;Badea, CT;Johnson, GA
通过将图像采集同步到多次呼吸的呼吸周期，并门控到心动周期，可以使用MR显微镜在活体啮齿动物中采集高分辨率的肺部和心脏图像。可以使图像采集与运动同步的精度定义了在这种研究中可以达到的最终分辨率极限。目前的工作是进行评估如何可靠的肺和心脏结构返回到同一位置，从呼吸到呼吸，心跳到心跳，跨越所需的长时间的MR显微镜。将不透射线珠通过手术粘合到麻醉机械通气大鼠的横膈膜腹部表面和心室上。我们使用数字显微放射照相术和两种特定的生物门控方法评价了珠子的运动范围（相对于参考椎体珠子）：1）通气同步采集，2）通气同步和心脏门控采集。位移的标准差（SD）为
One can acquire high-resolution pulmonary and cardiac images in live rodents with MR microscopy by synchronizing the image acquisition to the breathing cycle across multiple breaths, and gating to the cardiac cycle. The precision with which one can synchronize image acquisition to the motion defines the ultimate resolution limit that can be attained in such studies. The present work was performed to evaluate how reliably the pulmonary and cardiac structures return to the same position from breath to breath and beat to beat across the prolonged period required for MR microscopy. Radiopaque beads were surgically glued to the abdominal surface of the diaphragm and on the cardiac ventricles of anesthetized, mechanically ventilated rats. We evaluated the range of motion for the beads (relative to a reference vertebral bead) using digital microradiography with two specific biological gating methods: 1) ventilation synchronous acquisition, and 2) both ventilation synchronous and cardiac-gated acquisitions. The standard deviation (SD) of the displacement was