Glossopharyngeal Nerve Transection Impairs Unconditioned Avoidance of Diverse Bitter Stimuli in Rats
Glossopharyngeal Nerve Transection Impairs Unconditioned Avoidance of Diverse Bitter Stimuli in Rats
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DOI：
10.1037/a0023934
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发表时间：
2011-08-01
影响因子：
1.9
通讯作者：
Travers, Susan P.
中科院分区：
文献类型：
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作者：
Geran, Laura C.;Travers, Susan P.
越来越多的证据表明，在外周、中枢和行为水平上，对苦味刺激的反应存在异质性。例如，舌咽（GL）神经和接受其投射的神经元比鼓索（CT）神经对苦味刺激更敏感，这对于一些刺激GL的苦味刺激（如PROP和放线菌酮）尤其如此。鉴于这一信息，我们假设，削减GL将有一个更大的影响，行为回避放线菌酮和PROP比奎宁和地那铵，这也刺激CT，虽然在较低程度上比盐和酸。将40只雄性SD大鼠分为4组：双侧GL横断（GLX）组、鼓索横断（CTX）组、SHAM组和联合横断（CTX + GLX）组。术后回避功能产生的四个痛苦的刺激，使用一个刺激访问测试。对于所有刺激，与CTX和SHAM组相比，GLX显著损害回避（p <0.02），而CTX和SHAM组没有差异。与我们的假设相反，GLX对奎宁的影响大于放线菌酮（平均偏移为1.02 vs 0.27 log（10）单位）。此外，对于除放线菌酮以外的每种刺激，CTX + GLX联合横切比单独GLX更进一步地改变了浓度-反应函数（ps < .03），表明GSP神经能够在很大程度上保持对该刺激的回避。这一假设得到了环己酰亚胺反应细胞的报告支持，在NST和PBN中具有GSP神经支配的感受野。
There is growing evidence of heterogeneity among responses to bitter stimuli at the peripheral, central and behavioral levels. For instance, the glossopharyngeal (GL) nerve and neurons receiving its projections are more responsive to bitter stimuli than the chorda tympani (CT) nerve, and this is particularly true for some bitter stimuli like PROP & cycloheximide that stimulate the GL to a far greater extent. Given this information, we hypothesized that cutting the GL would have a greater effect on behavioral avoidance of cycloheximide and PROP than quinine and denatonium, which also stimulate the CT, albeit to a lesser degree than salts and acids. Forty male SD rats were divided into four surgery groups: bilateral GL transection (GLX), chorda tympani transection (CTX), SHAM surgery, and combined transection (CTX + GLX). Postsurgical avoidance functions were generated for the four bitter stimuli using a brief-access test. GLX significantly compromised avoidance compared to both CTX and SHAM groups for all stimuli (p < .02), while CTX and SHAM groups did not differ. Contrary to our hypothesis, GLX had a greater effect on quinine than cycloheximide (mean shift of 1.02 vs. 0.27 log(10) units). Moreover, combined CTX + GLX transection shifted the concentration-response function further than GLX alone for every stimulus except cycloheximide (ps < .03), suggesting that the GSP nerve is capable of maintaining avoidance of this stimulus to a large degree. This hypothesis is supported by reports of cycloheximide-responsive cells with GSP-innervated receptive fields in the NST and PBN.