Nanopores: Generation, Engineering, and Single-Molecule Applications
Nanopores: Generation, Engineering, and Single-Molecule Applications
复制标题
DOI：
10.1007/978-0-387-76497-9_11
复制
发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Siwy, Zuzanna
中科院分区：
文献类型：
--
作者：
Howorka, Stefan;Siwy, Zuzanna
基于离子孔电流的暂时阻塞，纳米孔能够感知单个分子。最初是在十年前用生物蛋白质孔进行的，现在常规的是用雕刻成聚合物和无机膜的人造孔进行电子记录。在通道工程的帮助下，分析物的范围已经从核酸扩大到多肽、蛋白质、有机聚合物和小分子。除了是一种有吸引力的分析方法外，纳米孔记录已经发展成为一种通用的平台技术，利用它可以研究单个分子的生物物理、物理化学和化学。纳米孔还可以用于分离技术和纳米流体，因为它们能够控制溶剂化离子的流动。与原子力和荧光显微镜的结合使用正在扩大纳米孔在单分子研究中的多功能性。
Nanopores enable the sensing of individual molecules based on the temporary blockades in ionic pore Current. Initially conducted a decade ago with a biological protein pore, electrical recordings are now routinely performed with synthetic pores sculptured into polymeric and inorganic membranes. Assisted by channel engineering, the range of analytes has been expanded from nucleic acids to peptides, proteins, organic polymers, and small molecules. Apart from being an attractive analytical approach, nanopore recording has developed into a general platform technology with which it is possible to examine the biophysics, physicochemistry, and chemistry of individual molecules. Nanopores can also be exploited for separation technologies and nanofluidics due to their ability to control the flow of solvated ions. The combined use with atomic force and fluorescence microscopy is extending the versatility of nanopores for single-molecule research.