IN-VIVO OXIMETRY USING EPR AND INDIA INK
IN-VIVO OXIMETRY USING EPR AND INDIA INK
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DOI：
10.1002/mrm.1910330214
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发表时间：
1995-02-01
影响因子：
3.3
通讯作者：
SWARTZ, HM
中科院分区：
文献类型：
--
作者：
GODA, F;LIU, KJ;SWARTZ, HM
电子顺磁共振（ERR）血氧仪的最新进展已经建立了使用颗粒材料云云石和酞菁锂（LiPc）作为探针来测量体内组织中的氧张力，具有灵敏度和准确性，与研究病理生理过程相关。印度墨水是另一种潜在的非常有用的EPR氧饱和度新探针，它具有云石和LiPc的关键特性，并且具有已经在临床上广泛使用的额外优势，没有明显的毒性。线宽对小于30mmhg的氧张力变化特别敏感；在此范围内，pO(2)的线宽/单位远大于云母石和LiPc。在体内可能发生的生物条件范围内，印度油墨的EPR光谱对pO(2)的响应与pH、氧化剂、还原剂和介质的性质无关。在本文中，我们描述了印度墨水的相关特性及其在实验动物和人类体内测量pO(2)的用途。
Recent advances in electron paramagnetic resonance (ERR) oximetry have established the use of the particulate materials fusinite and lithium phthalocyanine (LiPc) as probes for measurement of oxygen tensions in tissues in vivo with a sensitivity and accuracy that is relevant for studying pathophysiological processes. India ink, another potentially very useful new probe for EPR oximetry, shares the critical properties of fusinite and LiPc and has the additional advantage of already having been widely used clinically with no apparent toxicity. The line width is particularly sensitive to changes in oxygen tension of less than 30 mmHg; in this range the line broadening/unit of pO(2) is much greater than that of fusinite and LiPc. Over the range of biological conditions that can occur in vivo, the response of the EPR spectrum of India ink to pO(2) is independent of pH, oxidants, reductants, and the nature of the medium. In this paper we describe the relevant properties of India ink and its use to measure pO(2) in vivo in experimental animals and a human subject.