DNA sequence and regional assignment of the human follicle-stimulating hormone beta-subunit gene to the short arm of human chromosome 11.
DNA sequence and regional assignment of the human follicle-stimulating hormone beta-subunit gene to the short arm of human chromosome 11.
复制标题
人类卵泡刺激素β亚基基因的DNA序列和人类11号染色体短臂的区域分配。
DOI：
10.1089/dna.1987.6.205
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Shows,TB
中科院分区：
文献类型：
--
作者：
Watkins,PC;Eddy,R;Beck,AK;Vellucci,V;Leverone,B;Tanzi,RE;Gusella,JF;Shows,TB
分析了λ噬菌体中含有人卵泡刺激素(β-β)亚单位基因fshB的基因组片段，并测定了该克隆编码fsh-β的核苷酸序列。用含有67%λ-β编码序列的FSHB噬菌体亚克隆作为杂交探针，确定FSHB的人类染色体位置。用FSHBs探针筛选了一组含有人类染色体数目减少的鼠-人体细胞杂交细胞，在所有26个细胞杂交细胞中观察到FSHBs与人11号染色体完全分离。对一组含有11号染色体易位衍生品的细胞杂交的分析进一步将FSB定位到人类染色体区域11pll.2→11。λ噬菌体的另一个亚克隆检测到的Hind III限制性片段长度多态性现在为人类基因组的这一区域提供了一个遗传标记。
A human genomic DNA fragment in phage λ containingFSHB, the gene for the β-subunit of human follicle-stimulating hormone (FSH-β), was analyzed and the nucleotide sequence of the region of the clone encoding FSH-β was determined. A subclone of the λ phage containing 67% of FSH-β coding sequence was used as hybridization probe to determine the human chromosomal location ofFSHB. A panel of mouse-human somatic cell hybrids containing reduced numbers of human chromosomes was screened with theFSHBprobe; complete cosegregation ofFSHBwith human chromosome 11 was observed in all 26 cell hybrids tested. Analysis of a set of cell hybrids containing translocated derivatives of chromosome 11 further localizedFSHBto the human chromosome region llpll.2→11pter. AHind III restriction fragment length polymorphism (RFLP) detected by another subclone of the λ phage containingFSHBnow provides a genetic marker for this region of the human genome.