Expression of dihydropyridine binding sites in renal epithelial cells.
Expression of dihydropyridine binding sites in renal epithelial cells.
复制标题
肾上皮细胞中二氢吡啶结合位点的表达。
DOI：
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
N. Karin
中科院分区：
文献类型：
--
作者：
R. O'neil;J. M. Reid;R. Williams;N. Karin
It has recently been shown that rabbit kidney epithelial cells (proximal tubule) possess two dihydropyridine (DHP)-sensitive calcium entry channels (1, 2). To evaluate the properties of the DHP binding sites, the binding of the DHP, [3H]PN200-110, was studied in epithelial cell membrane fractions (proximal tubule) of rabbit kidney cortex. High-affinity binding sites for the DHP were observed in both basolateral and apical membranes and in a membrane microsomal preparation from rabbit primary cultures of proximal tubule cells (cultured PT). In an extended analysis of the basolateral membrane preparation, two high-affinity binding sites were evident with binding dissociation constants, Kd, of 0.005 and 0.75 nM. The Kd values are similar to that observed for L-type calcium channel alpha1-subunits. Using a homology-based cloning strategy, a 388-base fragment of an alpha1-subunit was cloned from RNA isolated from rabbit cultured PT cells and freshly isolated proximal tubules and found to encode a protein identical to the cardiac form of the L-type alpha1-subunit (alpha1C-subunit). It is concluded that renal epithelial cells express high-affinity dihydropyridine receptors and that the receptors may be components of plasma membrane calcium channels, including L-type calcium channels, that control calcium entry in these cells.
[3H]PN200-110 和 [3H]ryanodine 与骨骼肌膜的结合以及离子通道活性的重建。
影响因子：
2.9
作者：
Hamilton,SL;Alvarez,RM;Fill,M;Hawkes,MJ;Brush,KL;Schilling,WP;Stefani,E
通讯作者：
Stefani,E
从牛气管上皮中分离运输质膜囊泡。
DOI：
10.1016/0005-2736(83)90024-x
发表时间：
1983
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Langridge-Smith,JE;Field,M;Dubinsky,WP
通讯作者：
Dubinsky,WP