Methylation-Specific PCR
Methylation-Specific PCR
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DOI：
10.1007/978-1-61779-316-5_3
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发表时间：
2011-01-01
期刊：
影响因子：
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通讯作者：
Park, Jae-Gahb
中科院分区：
文献类型：
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作者：
Ku, Ja-Lok;Jeon, You-Kyung;Park, Jae-Gahb
启动子富CpG区（CpG岛）的DNA甲基化模式与哺乳动物细胞基因表达调控有关。启动子区CpG二核苷酸的过度甲基化抑制基因表达。尤其是在肿瘤中，基因沉默是通过肿瘤抑制基因启动子的异常甲基化而发生的。甲基化特异性PCR（MSP）是一种分析CpG岛DNA甲基化模式的方法。为了进行MSP，通过两个引物对修饰DNA并用两个引物对进行PCR，这两个引物对分别是可检测的甲基化和未甲基化DNA。MSP是一种快速评估CpG岛甲基化状态的方法。
DNA methylation patterns in CpG-rich regions of promoter, CpG islands, are concerned in regulation of gene expression in mammalian cells. Excessive methylation of CpG dinucleotides in promoter represses the gene expression. In cancer, especially, gene silencing is occurred through aberrant methylation in promoter of tumor suppressor genes. Methylation-specific PCR (MSP) is a method for analysis of DNA methylation patterns in CpG islands. For performing MSP, DNA is modified by and PCR performed with two primer pairs, which are detectable methylated and unmethylated DNA, respectively. MSP is a rapid measure for assession of the methylation status in CpG island.