Type III protein secretion is associated with death in lower respiratory and systemic Pseudomonas aeruginosa infections
Type III protein secretion is associated with death in lower respiratory and systemic Pseudomonas aeruginosa infections
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DOI：
10.1086/320737
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发表时间：
2001-06-15
影响因子：
6.4
通讯作者：
Wiener-Kronish, JP
中科院分区：
文献类型：
--
作者：
Roy-Burman, A;Savel, RH;Wiener-Kronish, JP
据报道，铜绿假单胞菌能够利用 III 型介导的途径分泌特定毒素。为了确定这种表型与人类疾病的关联，使用免疫印迹分析来检测从 108 名连续患者的呼吸道或血培养物中分离的铜绿假单胞菌中 III 型分泌蛋白的表达。当 III 型分泌蛋白 ExoS、ExoT、ExoU 或 PcrV 表达时，死亡的相对风险增加 6 倍。在细胞和小鼠模型中，表型与毒性独立相关。这种表型在急性感染患者中的患病率明显高于慢性感染囊性纤维化患者。这些结果表明，III 型蛋白分泌系统对于铜绿假单胞菌毒力的增加是不可或缺的。阳性表型是不良临床结果的预测因子。将来，此类分析可能有助于区分潜在致命的感染和定植，并有助于为危重患者确定适当的治疗方法。
The ability of Pseudomonas aeruginosa to secrete specific toxins using the type III-mediated pathway has been reported. To determine the association of this phenotype with human illness, immunoblot analysis was used to detect expression of type III secretory proteins in P. aeruginosa isolates from respiratory tract or blood cultures of 108 consecutive patients. Relative risk of mortality was 6-fold greater with expression of the type III secretory proteins ExoS, ExoT, ExoU, or PcrV. Phenotype was independently correlated with toxicity in cellular and murine models. Prevalence of this phenotype was significantly higher in acutely infected patients than in chronically infected patients with cystic fibrosis. These results suggest that the type III protein secretion system is integral to increased P. aeruginosa virulence. A positive phenotype is a predictor of poor clinical outcome. In the future, such analyses may help distinguish potentially lethal infection from colonization and help determine appropriate therapy for critically ill patients.