Promotion of monolayer formation in cultured whole pancreatic islets by 3-isobutyl-1-methylxanthine.
Promotion of monolayer formation in cultured whole pancreatic islets by 3-isobutyl-1-methylxanthine.
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3-异丁基-1-甲基黄嘌呤促进培养的全胰岛的单层形成。
DOI：
10.1073/pnas.75.4.1897
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发表时间：
1978
影响因子：
11.1
通讯作者：
D. Steiner
中科院分区：
文献类型：
--
作者：
H. Ohgawara;R. Carroll;C. Hofmann;C. Takahashi;M. Kikuchi;A. Labrecque;Y. Hirata;D. Steiner
正常成年大鼠胰岛通常保持完整和封装，即使在许多天的文化。相比之下，在磷酸二酯酶抑制剂3-异丁基-1-甲基黄嘌呤（0.1 mM）的存在下培养的胰岛更容易附着在塑料培养皿的表面，几乎均匀地形成内分泌细胞的单层。这种作用的机制尚不清楚，但可能涉及细胞环AMP含量的增加。成纤维细胞的生长似乎没有受到抑制剂的刺激。这些成人胰腺内分泌单层培养物可以很容易地产生，并为进一步的形态学和生物化学研究影响胰岛细胞的分化，生长和再生能力的因素提供有用的准备。
Normal adult rat islets usually remained intact and encapsulated, even after many days in culture. In contrast, islets cultured in the presence of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (0.1 mM) attached more readily to the surface of plastic culture dishes and almost uniformly formed monolayers of endocrine cells. The mechanism of this effect is not known but presumably involves increases in cellular cyclic AMP content. Fibroblast growth did not appear to be stimulated by the inhibitor. These adult pancreatic endocrine monolayer cultures can be produced readily and provide useful preparations for further morphological and biochemical studies of factors affecting the differentiation, growth, and regenerative capacity of islet cells.