Expression of the Na+/glucose co-transporter (SGLT1) in the intestine of domestic and wild ruminants
Expression of the Na+/glucose co-transporter (SGLT1) in the intestine of domestic and wild ruminants
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DOI：
10.1007/s004240000404
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发表时间：
2000-11-01
影响因子：
4.5
通讯作者：
Shirazi-Beechey, SP
中科院分区：
文献类型：
--
作者：
Wood, IS;Dyer, J;Shirazi-Beechey, SP
在从前反刍动物向成熟反刍动物状态过渡期间，评估了从食用草和粗饲料（GR）的反刍动物（绵羊和奶牛）肠道分离的刷状缘膜囊泡（BBMV）中Na+/葡萄糖共转运蛋白（SGLT 1）的活性和丰度。还比较了相同动物肠组织中SGLT 1信使核糖核酸（mRNA）的丰度。SGLT 1活性和表达的显著发育下降似乎是GR消耗反刍动物的典型特征，并且与内腔单糖水平的显著下降一致。绵羊SGLT 1互补脱氧核糖核酸（cDNA）在非洲爪蟾卵母细胞中的表达证实了分离的cDNA编码功能性Na+/葡萄糖共转运蛋白。牛肠SGLT 1蛋白序列（氨基酸347-658）的测定表明与羊SGLT 1蛋白具有99%的相似性，但羧基末端存在差异。与GR消耗反刍动物相反，SGLT 1蛋白和SGLT 1 mRNA的丰度在中间混合（IM）喂养山羊和休闲鹿和浓缩物选择（CS）驼鹿和鹿的反刍动物的肠道中仍然显着较高，这些饮食组与肠腔中单糖的可用性相关。
The activity and abundance of the Na+/glucose co-transporter (SGLT1) was assessed in brush-border-membrane vesicles (BBMV) isolated from the intestine of grass- and roughage- (GR) consuming ruminants (sheep and dairy cattle), during the transition from the pre-ruminant to the mature ruminant state. The abundance of SGLT1 messenger ribonucleic acid (mRNA) was also compared in the intestinal tissue of the same animals. The dramatic developmental decline in the activity and expression of SGLT1 appears to be typical of GR-consuming ruminants and is coincident with the significant decline in the levels of lumenal monosaccharides. Expression of the ovine SGLT1 complementary deoxyribonucleic acid (cDNA) in Xenopus laevis oocytes confirmed that the isolated cDNA encodes for a functional Na+/glucose co-transporter. Determination of a bovine intestinal SGLT1 protein sequence (amino acids 347-658) indicated 99% similarity to the ovine SGLT1 protein with differences in the carboxyl terminus. In contrast to GR-consuming ruminants, the abundance of SGLT1 protein and SGLT1 mRNA remained significantly high in the intestine of ruminants in both the intermediate-mixed (IM) feeding goat and fallow deer and the concentrate-selecting (CS) moose and roe deer, dietary groups correlating with the availability of monosaccharides in the intestinal lumen.