Triggering of high-speed neurite outgrowth using an optical microheater.
Triggering of high-speed neurite outgrowth using an optical microheater.
复制标题
使用光学微调器触发高速神经突生长。
DOI：
10.1038/srep16611
复制
发表时间：
2015-11-16
影响因子：
4.6
通讯作者：
Ishiwata S
中科院分区：
文献类型：
--
作者：
Oyama K;Zeeb V;Kawamura Y;Arai T;Gotoh M;Itoh H;Itabashi T;Suzuki M;Ishiwata S
光学微加热是一种强大的非侵入性方法，用于操纵生物功能，如基因表达，肌肉收缩和细胞兴奋。在这里，我们展示了其调节神经突生长的潜在用途。我们发现，光学微加热与水吸收1,455纳米激光束触发定向和爆炸性的神经突生长和分支在大鼠海马神经元。显微镜下聚焦的激光束将局部温度从36 ℃迅速升高到41 ℃（2 s内稳定），导致神经突在1 min内伸长超过10 μm。微管和肌动蛋白聚合的抑制剂抑制了神经突的这种高速、持续伸长，表明这些细胞骨架的热敏动力学在这种热诱导的神经突生长中起着至关重要的作用。此外，我们发现，微加热诱导损伤的神经突的再生和神经突的互连。这些结果证明了光学微加热方法用于构造任意神经网络的有效性。
Optical microheating is a powerful non-invasive method for manipulating biological functions such as gene expression, muscle contraction, and cell excitation. Here, we demonstrate its potential usage for regulating neurite outgrowth. We found that optical microheating with a water-absorbable 1,455-nm laser beam triggers directional and explosive neurite outgrowth and branching in rat hippocampal neurons. The focused laser beam under a microscope rapidly increases the local temperature from 36 °C to 41 °C (stabilized within 2 s), resulting in the elongation of neurites by more than 10 μm within 1 min. This high-speed, persistent elongation of neurites was suppressed by inhibitors of both microtubule and actin polymerization, indicating that the thermosensitive dynamics of these cytoskeletons play crucial roles in this heat-induced neurite outgrowth. Furthermore, we showed that microheating induced the regrowth of injured neurites and the interconnection of neurites. These results demonstrate the efficacy of optical microheating methods for the construction of arbitrary neural networks.