Mutational analysis of the Drosophila miniature-dusky (m-dy) locus: effects on cell size and circadian rhythms.
Mutational analysis of the Drosophila miniature-dusky (m-dy) locus: effects on cell size and circadian rhythms.
复制标题
果蝇微型暗色（m-dy）基因座的突变分析：对细胞大小和昼夜节律的影响。
DOI：
10.1093/genetics/128.3.571
复制
发表时间：
1991
期刊：
影响因子：
3.3
通讯作者：
Jackson,FR
中科院分区：
文献类型：
--
作者：
Newby,LM;White,L;DiBartolomeis,SM;Walker,BJ;Dowse,HB;Ringo,JM;Khuda,N;Jackson,FR
已进行突变分析，以探讨果蝇miniature-dusky（m-dy）基因座的功能。该位点的突变影响翅膀发育、生育力和行为。13个不同突变的遗传特征表明，m和dy变异体是一个单一复杂基因的等位基因。所有这些突变都改变了翅膀的大小，显然是通过减少发育中翅膀的单个表皮细胞的体积。在m突变体中，表皮细胞边界在成熟翅中持续存在，而在野生型或dy果蝇中，它们通常在羽化后1-2小时退化。这使得几个m突变体之间的细胞大小差异的直接可视化。m-dy基因座的突变也会影响行为过程。三个九个dy等位基因（dyn 1，dyn 3和dyn 4）延长昼夜活动和羽化节律的周期约1.5小时。相反，m突变体有正常的昼夜周期，但异常大的百分比的个人表达非周期性发作的活动。这些行为遗传学研究还表明，一种被称为Andante的现有“节奏”突变是m-dy基因座的等位基因。某些m-dy突变对翅膀和行为表型的不同影响表明，该基因座内存在可分离的功能域。
A mutational analysis has been performed to explore the function of the Drosophila melanogaster miniature-dusky (m-dy) locus. Mutations at this locus affect wing development, fertility and behavior. The genetic characterization of 13 different mutations suggests that m and dy variants are alleles of a single complex gene. All of these mutations alter wing size, apparently by reducing the volume of individual epidermal cells of the developing wing. In m mutants, epidermal cell boundaries persist in the mature wing, whereas they normally degenerate 1-2 hr after eclosion in wild-type or dy flies. This has permitted the direct visualization of cell size differences among several m mutants. Mutations at the m-dy locus also affect behavioral processes. Three out of nine dy alleles (dyn1, dyn3 and dyn4) lengthen the circadian period of the activity and eclosion rhythms by approximately 1.5 hr. In contrast, m mutants have normal circadian periods, but an abnormally large percentage of individuals express aperiodic bouts of activity. These behavior genetic studies also indicate that an existing "rhythm" mutation known as Andante is an allele of the m-dy locus. The differential effects of certain m-dy mutations on wing and behavioral phenotypes suggest that separable domains of function exist within this locus.