Protein-protein interaction at crystal contacts
Protein-protein interaction at crystal contacts
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DOI：
10.1002/prot.340230413
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发表时间：
1995-12-01
期刊：
影响因子：
--
通讯作者：
Rodier, F
中科院分区：
文献类型：
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作者：
Janin, J;Rodier, F
填充接触是晶体产物，但它们利用了与控制蛋白质-蛋白质复合物和低聚蛋白质的特定识别相同的力。他们提供了非特异性蛋白质-蛋白质相互作用的例子，可以与生物学相关的相互作用进行比较。我们评估了152种晶体形式中不对称单元包含单体蛋白质的成对界面的数量和大小，在那些没有a-fold对称元素的晶体形式中，我们发现分子形成8到10个成对界面。每个分子表面的覆盖面积很大，可达4400埃(2)。成对界面埋藏200-1200埃(2)，类似于计算机模拟中随机生成的界面，而蛋白酶抑制剂或抗原-抗体复合物的界面埋藏1500埃(2)或更多。因此，在这种配合物中发生的特定接触延伸到比非特定接触更大的表面上。在具有a-折叠对称的晶体形式中，平均成对界面比没有a-折叠对称的晶体形式更少和更大，一些隐藏1500-2500埃(2)，就像低聚蛋白质中的界面一样，并产生可能在结晶之前在溶液中形成的“晶体低聚物”。(C) 1995 Wiley-Liss, Inc。
Packing contacts are crystal artifacts, yet they make use of the same forces that govern specific recognition in protein-protein complexes and oligomeric proteins. They provide examples of a nonspecific protein-protein interaction which can be compared to biologically relevant ones, We evaluate the number and size of pairwise interfaces in 152 crystal forms where the asymmetric unit contains a monomeric protein, In those crystal forms that have no element of a-fold symmetry, we find that molecules form 8 to 10 pairwise interfaces. The total area of the surface buried on each molecule is large, up to 4400 Angstrom(2). Pairwise interfaces bury 200-1200 Angstrom(2), like interfaces generated at random in a computer simulation, and less than interfaces in protease-inhibitor or antigen-antibody complexes, which bury 1500 Angstrom(2) or more. Thus, specific contacts occurring in such complexes extend over a larger surface than nonspecific ones, In crystal forms with a-fold symmetry, pairwise interfaces are fewer and larger on average than in the absence of a-fold symmetry, Some bury 1500-2500 Angstrom(2), like interfaces in oligomeric proteins, and create ''crystal oligomers'' which may have formed in the solution before crystallizing. (C) 1995 Wiley-Liss, Inc.