Synthesis and application of boronic acid-immobilized porous polymer particles: A novel packing for high-performance liquid affinity chromatography
Synthesis and application of boronic acid-immobilized porous polymer particles: A novel packing for high-performance liquid affinity chromatography
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DOI：
10.1016/0378-4347(96)00006-0
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发表时间：
1996-04-26
期刊：
影响因子：
--
通讯作者：
Terauchi, K
中科院分区：
文献类型：
--
作者：
Koyama, T;Terauchi, K
研究了一种新型高效液相亲和色谱法测定糖化蛋白的填料的制备方法，并对其在糖尿病血清中的应用进行了基础性研究。为了定量糖化蛋白质，如糖化血清白蛋白，一种新的亲水性和耐用的多孔聚合物颗粒在我们的实验室最近开发的用作基本基质。以1，1 '-羰基二咪唑（CDI）为活化剂，对活化后的基质与间氨基苯硼酸半硫酸盐（APBA）的偶联反应进行了优化。发现APBA偶联产率的最佳值是在酸性条件下获得的，与以前工作者报道的数据非常不同。用此APBA固定化基质制备了亲和柱，并研究了其在糖化血清蛋白HPLC分离中的应用。本文还对糖尿病诊断的基础和初步结果进行了讨论。
A preparation method of a novel type of packing materials for high-performance liquid affinity chromatography to determine glycated proteins was studied, and a fundamental study on its application to diabetic serum was conducted. To quantify glycated proteins such as glycated serum albumin, a new hydrophilic and durable porous polymer particle recently developed in our laboratory was used as the basic matrix. The matrix was activated with 1,1'-carbonyldiimidazole (CDI), and optimization of the coupling reaction between these CDI-activated matrix and m-aminophenylboronic acid hemisulfate (APBA) as affinity ligand was investigated. The optimum value for the APBA coupling yield was found to be obtained under acidic conditions very different from the data reported by previous workers. Using this APBA-immobilized matrix an affinity column was prepared, and its usefulness in HPLC separation of glycated serum proteins was investigated. Also, the fundamental and preliminary results for diagnosis of diabetes mellitus are discussed in this paper.