Measurement of menadione in urine by HPLC.
Measurement of menadione in urine by HPLC.
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DOI：
10.1016/j.jchromb.2010.07.016
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发表时间：
2010-09-15
影响因子：
3
通讯作者：
Booth, Sarah L.
中科院分区：
文献类型：
--
作者：
Al Rajabi, Ala;Peterson, James;Choi, Sang-Woon;Suttie, John;Barakat, Susan;Booth, Sarah L.
Menadione is a metabolite of vitamin K that is excreted in urine. A high performance liquid chromatography (HPLC) method using a C30 column, post-column zinc reduction and fluorescence detection was developed to measure urinary menadione. The mobile phase was composed of 95% methanol with 0.55% aqueous solution and 5% DI H2O. Menaquinone-2 (MK-2) was used as an internal standard. The standard calibration curve was linear with a correlation coefficient (R2) of 0.999 for both menadione and MK-2. The lower limit of quantification (LLOQ) was 0.3 pmole menadione/mL urine. Sample preparation involved hydrolysis of menadiol conjugates and oxidizing the released menadiol to menadione. Using this method, urinary menadione was shown to increase in response to 3 years of phylloquinone supplementation. This HPLC method is a sensitive and reproducible way to detect menadione in urine. Research support: USDA ARS Cooperative Agreement 58-1950-7-707.
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DOI：
10.1002/bmc.1130020103
发表时间：
1987-02-01
期刊：
Biomedical chromatography : BMC
影响因子：
--
作者：
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通讯作者：
Sadowski, J A
影响因子：
4.4
作者：
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影响因子：
5.8
作者：
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通讯作者：
Dawson-Hughes, Bess
DOI：
10.1016/j.jchromb.2004.03.009
发表时间：
2004-06-15
影响因子：
3
作者：
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通讯作者：
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影响因子：
3.6
作者：
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通讯作者：
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