Characterization of human fetal cord blood steroid profiles in relation to fetal sex and mode of delivery using temperature-dependent inclusion chromatography and principal component analysis (PCA)
Characterization of human fetal cord blood steroid profiles in relation to fetal sex and mode of delivery using temperature-dependent inclusion chromatography and principal component analysis (PCA)
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DOI：
10.1016/j.jchromb.2007.05.041
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发表时间：
2007-08-15
影响因子：
3
通讯作者：
Zarzycki, Pawel K.
中科院分区：
文献类型：
--
作者：
Clifton, Vicki L.;Bisits, Andrew;Zarzycki, Pawel K.
在本工作中，人类男性和女性胎儿脐带血样品进行纯化，选择性提取和分离，以检查从极性雌四醇相对非极性孕酮使用固相萃取基于C-18管和β-环糊精驱动的温度依赖性包合色谱的一部分类固醇。所得UV二极管阵列色谱图显示存在27个峰。使用主成分分析，揭示了男性/女性和劳动/不劳动集群之间的差异UV检测类固醇的色谱模式进行了分析。9个确定的类固醇，包括：雌四醇，17 β-雌二醇，雌酮，雌三醇，皮质醇，可的松，孕酮，20 α-羟孕酮和17 α-羟孕酮的定量分析，男性和女性之间没有显着差异。雄性和雌性胎仔之间的显著差异与尚未鉴别的化合物有关。四个峰与分娩显著不同，对应于皮质醇、可的松和两种未鉴定的化合物。该方案可以区分使用单一类固醇或不同低分子量生物标志物的单变量测量值不容易识别的临床组之间的显著差异。此外，我们提供了新的证据，尽管没有睾酮，但男性和女性胎儿之间存在许多类固醇和低分子量化合物。(C)2007 Elsevier B. V.保留所有权利。
In the present work, human male and female fetal cord blood samples were purified, selectively extracted and separated to examine a fraction of steroids ranging from polar estetrol to relatively non-polar progesterone using solid phase extraction based on C-18 tubes and beta-cyclodextrin driven temperature dependent inclusion chromatography. Resulting UV diode array chromatographic patterns revealed the presence of 27 peaks. Chromatographic patterns of UV detected steroids were analyzed using principal components analysis which revealed differences between male/female and labour/not-in-labour clusters. Quantitative analysis of nine identified steroids including: estetrol, 17 beta-estradiol, estrone, estriol, cortisol, cortisone, progesterone, 20 alpha-hydroxyprogesterone and 17 alpha-hydroxyprogesterone were not significantly different between males and females. Significant differences between male and female fetuses were related to as yet unidentified compounds. Four peaks were significantly different with labour which corresponded with corti sol, cortisone and two unidentified compounds. This protocol may distinguish significant differences between clinical groups that are not readily identifiable using univariate measurements of single steroids or different low molecular mass biomarkers. Moreover, we have provided new evidence that despite the absence of testosterone there are number of steroids and low molecular mass compounds that differ between male and female fetuses. (C) 2007 Elsevier B.V. All rights reserved.