Viral and bacterial assemblage covariance in oligotrophic waters of the West Florida Shelf (Gulf of Mexico)
Viral and bacterial assemblage covariance in oligotrophic waters of the West Florida Shelf (Gulf of Mexico)
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DOI：
10.1017/s0025315406013506
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发表时间：
2006-06-01
影响因子：
1.2
通讯作者：
Wommack, KE
中科院分区：
文献类型：
--
作者：
Hewson, I;Wingett, DM;Wommack, KE
病毒被假设为通过调节分类群间竞争的结果而引起细菌群落的多样性增强。然而，伴随的文件中的病毒和细菌的组合物在贫营养沃茨是罕见的，特别是在原位随着时间的推移，几乎没有信息的时间变化virioplankton组合组合物在贫营养水体。组装组成的病毒（通过脉冲场凝胶电泳，PFGE）和细菌（通过，自动rRNA基因间间隔区分析，ARISA）进行了比较，在表面拉格朗日漂流部署在贫营养的墨西哥湾在2001年，2002年和2003年夏天。在垂直剖面上，病毒和细菌在沃茨水体中的丰度均达到最大值，并随深度的增加而降低，但其丰度在不同深度之间没有显著差异，表明生物量和多样性的独立性。病毒组合变化迅速（0.17-0.32 Jaccard指数d（-1）），这与已报道的表层沃茨中细菌组合的变化率相似。病毒和细菌的组合组成的模式显着相关（P < 0.001，r=0.58节点之间的行列），和两个组合聚类主要是由年，然后由深度。这些独立于培养的观察结果表明了病毒和细菌组合之间的关系，这些组合在开放的海水中是动态的。即使在相对较低的系统发育分辨率的ARISA和PFGE方法，结果支持的想法，病毒可能会影响宿主组合的物种组成。
Viruses are hypothesized to cause enhanced diversity in bacterial communities by regulating the outcome of intertaxon competition. However, concomitant documentation of viral and bacterial assemblage composition in oligotrophic waters are rare, particularly in situ over time, and there is almost no Information on the temporal variability in virioplankton assemblage composition in oligotrophic water masses. Assemblage composition of viruses (via pulsed-field gel electrophoresis, PFGE) and bacteria (Via, automated rRNA intergenic spacer analysis, ARISA) was compared during Surface lagrangian drifter deployments in the oligotrophic Gulf of Mexico during summer 2001, 2002, and 2003. 111 vertical profile, Viruses and bacteria both had Maximum abundances in surface waters, which decreased with depth; however, the richness of their assemblages was not significantly different between depths, suggesting independence of biomass and diversity Viral assemblages changed rapidly (0.17-0.32 Jaccard index d(-1)), which was similar to the rate of change in bacterial assemblages reported Surface waters. Patterns of viral and bacterial assemblage composition were significantly related (P < 0.001, r=0.58 between node ranks), and both assemblages clustered primarily by year and then by depth. These cultivation-independent observations demonstrate relationships between viral and bacterial assemblages, which are dynamic in patches of open ocean water. Even at the relatively low phylogenetic resolution of the ARISA and PFGE methods, the results support the idea that viruses may influence the species composition of host assemblages.