Twist is required for muscle template splitting during adult Drosophila myogenesis
Twist is required for muscle template splitting during adult Drosophila myogenesis
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DOI：
10.1006/dbio.1998.9040
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发表时间：
1998-11-01
影响因子：
2.7
通讯作者：
Olson, EN
中科院分区：
文献类型：
--
作者：
Cripps, RM;Olson, EN
基本螺旋-环-螺旋转录因子Twist是果蝇幼体和成体肌肉正常发育所必需的。成虫通常有6对背侧纵向间接夜肌(DLMS)，而当扭转功能减弱时，只形成3对DLMS。虽然Twist在整个幼虫和早期蛹阶段的成体肌肉前体中都有表达，但我们证明了Twist功能只有在幼虫晚期才是DLm模式所必需的。在野生型果蝇中，这是在三对持久的幼虫肌肉纤维纵向分裂形成六对DLMS的模板之前。通过观察蛹发育不同时期的切片，我们发现在扭转突变体中没有出现幼虫肌肉的分裂，这表明扭转功能是在幼虫分化之前诱导幼虫模板发生重大变化所必需的。Twist在幼虫肌肉分裂中的作用可能是由肌细胞增强因子-2(MEF2)介导的，因为在Mef2亚型突变体中，分裂也被减少，并且Mef2的表达依赖于Twist。我们的发现明确了Twist和MEF2在蛹肌发生中的特定作用，并证明了这些转录因子在成体肌肉前体细胞中发挥作用，调节控制肌肉细胞分裂和形态发生的下游因素。(C)1998年学术出版社。
The basic helix-loop-helix transcription factor Twist is required for normal development of larval and adult somatic muscles in Drosophila. Adult flies normally have six pairs of dorsal longitudinal indirect night muscles (DLMs), whereas when Twist function is reduced, only three pairs of DLMs are formed. Although twist is expressed in precursors of adult muscles throughout the larval and early pupal stages, we demonstrate that Twist function is required only during the late larval stage for DLM patterning. In wild-type flies, this is just prior to the time when three pairs of persistent larval muscle fibers split longitudinally to form templates for the six pairs of DLMs. By examining sections at various times during pupal development, we found that splitting of the larval muscles does not occur in twist mutants, indicating that Twist function is required to induce major changes in the larval templates prior to differentiation. The function of Twist in larval muscle splitting is likely mediated by myocyte enhancer factor-2 (MEF2) since in Mef2 hypomorphic mutants splitting is also reduced and Mef2 expression is dependent upon Twist. Our findings define specific roles for Twist and MEF2 during pupal myogenesis and demonstrate that these transcription factors function in adult muscle precursor cells to regulate downstream factors controlling muscle cell splitting and morphogenesis. (C) 1998 Academic Press.