A mitohormetic response to pro-oxidant exposure in the house mouse
A mitohormetic response to pro-oxidant exposure in the house mouse
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家鼠对促氧化剂暴露的线粒体激素反应
DOI：
10.1152/ajpregu.00176.2017
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Hood, Wendy R.
中科院分区：
文献类型：
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作者：
Zhang, Yufeng;Humes, Frances;Almond, Gregory;Kavazis, Andreas N.;Hood, Wendy R.
假设线粒体对活性氧 (ROS) 暴露表现出双相反应。在这项研究中，我们评估了家鼠 X 射线照射后线粒体性能和氧化应激的时间过程变化。 48 只小鼠平均分为 6 组，包括未受辐射的对照组和 5 个实验组，实验组在 X 射线照射和安乐死之间的时间各不相同（X 射线照射后 1 小时和 1、4、7 和 10 天）。我们测量了与线粒体呼吸功能相关的参数，以及离体肝脏和骨骼肌线粒体的 ROS 排放，以及肝脏、骨骼肌和心脏组织中氧化损伤和抗氧化剂的水平。 X 射线照射后，线粒体功能最初下降，但很快恢复，并在照射 10 天后升高。 X射线照射10天后，过氧化氢产生、脂质过氧化和蛋白质羰基化呈现倒U形曲线，水平恢复到对照或低于对照。酶促抗氧化剂和线粒体生物发生标记物在辐射后表现出组织特异性反应。这些数据首次按时间顺序描述了轻度辐射后的线粒体激素反应，并强调了细胞对 ROS 暴露所表现出的保护性反应。这项研究还为未来在多种生理环境中的线粒体和氧化应激研究提供了有价值的信息和应用。
Mitochondria are hypothesized to display a biphasic response to reactive oxygen species (ROS) exposure. In this study, we evaluated the time course changes in mitochondrial performance and oxidative stress in house mice following X-irradiation. Forty-eight mice were equally divided among six groups, including a nonirradiated control and five experimental groups that varied in time between X-ray exposure and euthanasia (1 h and 1, 4, 7, and 10 days after X-irradiation). We measured parameters associated with mitochondrial respiratory function and ROS emission from isolated liver and skeletal muscle mitochondria and levels of oxidative damage and antioxidants in liver, skeletal muscle, and heart tissues. Mitochondrial function dropped initially after X-irradiation but recovered quickly and was elevated 10 days after the exposure. Hydrogen peroxide production, lipid peroxidation, and protein carbonylation showed inverse U-shaped curves, with levels returning to control or lower than control, 10 days after X-irradiation. Enzymatic antioxidants and markers for mitochondrial biogenesis exhibited a tissue-specific response after irradiation. These data provide the first chronological description of the mitohormetic response after a mild dose of irradiation and highlight the protective response that cells display to ROS exposure. This study also provides valuable information and application for future mitochondrial and oxidative stress studies in numerous physiological settings.