Efficient propagation of archetype BK and JC polyomaviruses
Efficient propagation of archetype BK and JC polyomaviruses
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DOI：
10.1016/j.virol.2011.10.026
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发表时间：
2012-01-20
期刊：
影响因子：
3.7
通讯作者：
Imperiale, Michael J.
中科院分区：
文献类型：
--
作者：
Broekema, Nicole M.;Imperiale, Michael J.
BKPyV和JCPyV是密切相关的普遍存在的人类病原体，其在免疫功能低下的患者中引起疾病。调控区的DNA序列区分了这些病毒的两种形式，即原型病毒和重排病毒。尽管存在用于重排的BKPyV和JCPyV的细胞培养系统，但目前还没有稳健的细胞培养系统来研究原型病毒。大T抗原（TAg）是启动病毒DNA合成所需的病毒编码蛋白。因为原型病毒产生不可检测水平的TAg，我们假设TAg过表达将刺激原型病毒复制。在293TT细胞（过表达SV40 TAg的人胚肾细胞）中观察到BKPyV和JCPyV原型形式的有效繁殖。重要的是，在病毒生长过程中维持了调控区的原型结构。未观察到默克尔细胞、KI或WU多瘤病毒的显著复制。293TT细胞为详细研究提供了繁殖原型BKPyV和JCPyV的手段。(C)2011 Elsevier Inc. All rights reserved.
BKPyV and JCPyV are closely related, ubiquitous human pathogens that cause disease in immunocompromised patients. The DNA sequence of the regulatory regions distinguishes two forms of these viruses, designated archetype and rearranged. Although cell culture systems exist for rearranged BKPyV and JCPyV, currently there is no robust cell culture system to study the archetype viruses. Large T antigen (TAg) is a virally encoded protein required to initiate viral DNA synthesis. Because archetype virus produces undetectable levels of TAg, we hypothesized that TAg overexpression would stimulate archetype virus replication. Efficient propagation of the archetype forms of BKPyV and JCPyV was observed in 293TT cells, human embryonic kidney cells overexpressing SV40 TAg. Importantly, the archetypal structure of the regulatory region was maintained during viral growth. Significant replication was not observed for Merkel cell, KI, or WU polyomaviruses. 293TT cells provide a means of propagating archetype BKPyV and JCPyV for detailed study. (C) 2011 Elsevier Inc. All rights reserved.