A modified live PRRSV vaccine and the pathogenic parent strain induce regulatory T cells in pigs naturally infected with Mycoplasma hyopneumoniae
A modified live PRRSV vaccine and the pathogenic parent strain induce regulatory T cells in pigs naturally infected with Mycoplasma hyopneumoniae
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DOI：
10.1016/j.vetimm.2010.12.011
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发表时间：
2011-04-15
影响因子：
1.8
通讯作者：
Pelzer, K. D.
中科院分区：
文献类型：
--
作者：
LeRoith, T.;Hammond, S.;Pelzer, K. D.
猪繁殖与呼吸综合征病毒（PRRSV）疫苗缺乏异源保护是目前该领域的一个主要问题。 PRRS 疫苗的异源保护取决于疫苗诱导干扰素γ (IFN-γ) 反应的能力。病毒逃避免疫系统的一种机制是激活调节性 T 细胞 (T-reg)，从而诱导白介素 10 而不是 IFN-γ 的诱导。我们的假设是，目前的 PRRS 疫苗在诱导 T-reg 的能力方面与致病毒株没有区别，这一假设是通过给三组猪接种两种病原病毒和减毒疫苗株并评估外周血单核细胞中 T-reg 的数量来测试的。在接种之前，尽管猪已接种疫苗，但在运送到我们的研究设施之前，猪仍自然感染猪肺炎支原体。我们的结果表明，PRRSV 疫苗株和亲本株同样能够在自然感染猪肺炎支原体的猪中诱导 T-reg。疫苗组和 PRRSV 组的猪的肺部病变评分高于对照组的猪。结果表明，猪肺炎支原体呼吸道疾病的恶化可能是由于PRRSV疫苗接种和病毒感染能够诱导调节性T细胞的能力所致。 (C) 2011 Elsevier B.V. 保留所有权利。
The lack of heterologous protection by porcine reproductive and respiratory syndrome virus (PRRSV) vaccines is currently a major problem in the field. Heterologous protection by PRRS vaccines depends on the ability of the vaccine to induce an interferon gamma (IFN-gamma) response. One mechanism by which the virus evades the immune system is by activating regulatory T cells (T-regs), resulting in induction of interleukin 10 rather than IFN-gamma. Our hypothesis that current PRRS vaccines do not differ from pathogenic strains in the ability to induce T-regs was tested by inoculating three groups of pigs with two pathogenic viruses and an attenuated vaccine strain and evaluating the number of T-regs in peripheral blood mononuclear cells. Before inoculation, the pigs, although vaccinated became infected naturally with Mycoplasma hyopneumoniae before shipment to our research facility. Our results show that the PRRSV vaccine strain and parent strain are equally able to induce T-regs in pigs naturally infected with M. hyopneumoniae. Pigs in the vaccine and PRRSV groups had higher lung lesion scores than pigs in the control groups. The results suggest that the exacerbation M. hyopneumoniae respiratory disease may be due to the ability of PRRSV vaccination and viral infection to induce regulatory T cells. (C) 2011 Elsevier B.V. All rights reserved.