Structural studies of Ets-1/Pax5 complex formation on DNA
Structural studies of Ets-1/Pax5 complex formation on DNA
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DOI：
10.1016/s1097-2765(01)00410-5
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发表时间：
2001-12-01
期刊：
影响因子：
16
通讯作者：
Wolberger, C
中科院分区：
文献类型：
--
作者：
Garvie, CW;Hagman, J;Wolberger, C
Pax 5与转录激活因子Ets家族成员一起调节mb-1基因的B细胞特异性表达。Ets蛋白本身与Pax 5/Ets结合位点结合较差，但可以通过与Pax 5的协同相互作用被募集到该位点。Ets-1的ETS结构域和Pax 5与DNA结合的配对结构域的结构揭示了Pax 5选择性募集不同Ets蛋白的分子细节。与Ets-1单独与高亲和力和低亲和力DNA位点结合的结构比较表明，Pax 5改变了Ets-1与DNA的接触。一种蛋白质改变另一种蛋白质的DNA序列特异性接触的能力为转录的组合调节提供了一般机制。
Pax5 regulates the B cell-specific expression of the mb-1 gene together with members of the Ets family of transcriptional activators. The Ets proteins on their own bind poorly to the Pax5/Ets binding site, but can be recruited to the site by cooperative interactions with Pax5. The structure of the ETS domain of Ets-1 and the paired domain of Pax5 bound to DNA reveals the molecular details of the selective recruitment of different Ets proteins by Pax5. Comparison with structures of Ets-1 alone bound to both high- and low-affinity DNA sites reveals that Pax5 alters the Ets-1 contacts with DNA. The ability of one protein to alter the DNA sequence-specific contacts of another provides a general mechanism for combinatorial regulation of transcription.