Mouse male sterility and histoincompatibility (mshi) maps between the D10Mit51/168/212 cluster and D10Mit213.
Mouse male sterility and histoincompatibility (mshi) maps between the D10Mit51/168/212 cluster and D10Mit213.
复制标题
D10Mit51/168/212 簇和 D10Mit213 之间的小鼠雄性不育和组织不相容性 (mshi) 图谱。
DOI：
10.1007/s003359901021
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
King,TR
中科院分区：
文献类型：
--
作者：
Rule,MC;Mutcherson2nd,RJ;Foss,AD;Nguyen,TK;Myrie,KA;King,TR
小鼠隐性雄性不育和组织不亲和性突变对移植和雄性生殖具有多效性。先前对回交小鼠进行的分析，通过睾丸形态学或同种异体移植排斥反应将每种性状定位于近端小鼠Chr 10的20 cM区域。在这里，我们提出了一个新的276个成员的种内回交小组的微卫星多态性分析，包括一组135个男性类型的不育和组织不相容性，这两个特点的地方控制mshi在1.7 cM的间隔标记D10 Mit 51/168/212和D10 Mit 213。此外，该分析允许对themshilocus的双基因模型进行明确的测试，并提供了对两种男性中themshi产生的组织发生表型的突变率的测量，（88.4 ± 3.9%）和雌性（91.0 ± 3.5%）。所呈现的精细结构图应有助于在该区域进行染色体游走，并最终对受该有趣突变影响的基因进行分子鉴定。
The recessive male sterility and histoincompatibility (mshi) mutation in the mouse generates pleiotropic effects on graft transplantation and male reproduction. Previous analysis of backcross mice typed formshieither by testicular morphology or by allograft rejection has located each trait to a 20-cM region on proximal mouse Chr 10. Here we present the microsatellite polymorphism analysis of a new 276-member intraspecific backcross panel—including a set of 135 males typed for sterility and histoincompatibility—that places both features controlled bymshiwithin a 1.7-cM interval between markersD10Mit51/168/212andD10Mit213.In addition, this analysis has allowed an explicit test of a two-gene model for themshilocus and has provided a measurement of the penetrance of themshi-generated histogenic phenotype in both male (88.4 ± 3.9%) and female (91.0 ± 3.5%) mutants. The fine-structure map presented should facilitate a chromosome walk across this region and, ultimately, the molecular identification of the gene or genes affected by this interesting mutation.