Comparative effects of ethylene inhibitors on Agrobacterium-mediated transformation of drought-tolerant wild watermelon.
Comparative effects of ethylene inhibitors on Agrobacterium-mediated transformation of drought-tolerant wild watermelon.
复制标题
乙烯抑制剂对农杆菌介导的耐旱野生西瓜转化的影响比较。
DOI：
10.1080/09168451.2018.1431516
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Malambane;G.;Nonaka;S.;Shiba;H.;Ezura;H.;Tsujimoto;H.;Akashi;K.
乙烯（C2 H4）是一种植物激素，在非生物胁迫和生物胁迫下产生，是影响农杆菌介导转化效率的重要因素。本研究比较了不同乙烯抑制剂对农杆菌介导的耐旱野生西瓜遗传转化效率的影响。因此，与化学抑制剂如AgNO 3和氨基乙氧基乙烯基甘氨酸（AVG）的应用相比，当感染的农杆菌含有1-氨基环丙烷羧酸（ACC）脱氨酶（acdS）基因时，观察到较低的乙烯水平，所述1-氨基环丙烷羧酸（ACC）脱氨酶（acdS）将乙烯前体ACC裂解为α-酮丁酸和氨。GUS组织化学和分光光度酶法表明acds比化学乙烯抑制剂更能促进基因转移。在acdS和AVG处理的外植体中，转基因芽的形成效率较高。这些观察结果表明，在共培养和芽形成过程中控制乙烯水平，特别是使用携带acds的农杆菌，有利于提高该植物的转化效率。
Ethylene (C2H4), a phytohormone that is produced in response to both abiotic and biotic stresses, is an important factor influencing the efficiency ofAgrobacterium-mediated transformation. In this study, effects of various ethylene inhibitors on the efficiency ofAgrobacterium-mediated genetic transformation in drought-tolerant wild watermelon was comparatively examined. Consequently, in comparison to the application of chemical inhibitors such as AgNO3and aminoethoxyvinylglycine (AVG), lower ethylene level was observed when the infectingAgrobacteriumcontained a gene for 1-aminocyclopropane-carboxylic acid (ACC) deaminase (acdS), which cleaves ethylene precursor ACC into α-ketobutyrate and ammonia. GUS histochemical and spectrophotometric enzyme assays showed thatacdSwas more effective in enhancing gene transfer than the chemical ethylene inhibitors. Efficiency of transgenic shoots formation was higher inacdS- and AVG-treated explants. These observations demonstrated that controlling the ethylene level during co-cultivation and shoot formation, particularly using theacdS-harboringAgrobacterium,is advantageous for enhancing the transformation efficiency in this plant.