Brain-derived neurotrophic factor: a novel human ovarian follicular protein.
Brain-derived neurotrophic factor: a novel human ovarian follicular protein.
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DOI：
10.1210/jcem.87.2.8213
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发表时间：
2002-02
期刊：
影响因子：
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通讯作者：
D. Seifer;Bo Feng;R. Shelden;Shiling Chen;C. Dreyfus
中科院分区：
文献类型：
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作者：
D. Seifer;Bo Feng;R. Shelden;Shiling Chen;C. Dreyfus
神经营养因子是一类可溶性多肽生长因子，在哺乳动物神经系统中发挥重要作用。脑源性神经营养因子（BDNF）最初被描述为神经系统中的一种神经营养因子，但现在已显示在包括内分泌组织在内的各种非神经元组织中表达。我们检测了人类卵泡可能分泌的BDNF，并进一步研究了小鼠卵母细胞，以确定BDNF对卵母细胞成熟的可能影响。在一系列的实验中，从体外受精后取卵的人类标本中，在人类卵泡液中检测到BDNF。为了确定BDNF的来源，将卵丘颗粒细胞（直接围绕发育中的卵母细胞的细胞）在细胞培养物中生长1-2天。BDNF蛋白在培养液中24小时内增加。此外，BDNF的释放在cAMP或forskolin（cAMP的激活剂）刺激后增强。相比之下，壁颗粒细胞（卵泡内衬细胞），卵母细胞和胚胎没有释放明显数量的BDNF。为了检查BDNF的可能靶点，使用小鼠研究来定位BDNF受体，Trk B，免疫细胞化学。该受体存在于分离的卵母细胞的表面上。此外，BDNF促进小鼠卵母细胞在培养中的成熟。这些实验首次证明了BDNF在人类卵巢卵泡细胞中的存在和分泌，并表明BDNF在卵母细胞成熟的调节和调制中可能起作用。
Neurotrophins are a family of soluble polypeptide growth factors widely recognized for their roles in the mammalian nervous system. One such neurotrophin, brain-derived neurotrophic factor (BDNF) was originally described in the nervous system but has now been shown to be expressed in a variety of nonneuronal tissues including endocrine tissues. We examined the human ovarian follicle for its possible secretion of BDNF and further studied mouse oocytes to determine BDNF's possible influence upon oocyte maturation. In a series of experiments derived from human specimens from in vitro fertilization following oocyte retrieval, BDNF was detected in human follicular fluid. To define the source of BDNF, cumulus granulosa cells (the cells that immediately surround the developing oocyte) were grown in cell culture for 1-2 d. BDNF protein increased over 24 h in the culture medium. Moreover, the release of BDNF was enhanced upon stimulation with cAMP or forskolin, an activator of cAMP. In contrast, mural granulosa (cells lining the follicle), oocytes, and embryos did not release appreciable quantities of BDNF. To examine possible targets of BDNF, mouse studies were used to localize the BDNF receptor, Trk B, immunocytochemically. The receptor was present on the surface of isolated oocytes. Moreover, BDNF promoted mouse oocyte maturation in culture. These experiments demonstrate for the first time the presence and secretion of BDNF from follicular cells in the human ovary and suggest a possible role for BDNF in the regulation and modulation of oocyte maturation.