rOmpA is a critical protein for the adhesion of Rickettsia rickettsii to host cells
rOmpA is a critical protein for the adhesion of Rickettsia rickettsii to host cells
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DOI：
10.1006/mpat.1997.0197
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发表时间：
1998-05-01
影响因子：
3.8
通讯作者：
Walker, DH
中科院分区：
文献类型：
--
作者：
Li, H;Walker, DH
rOmpA和rOmpB是立克次体的免疫显性的表面暴露蛋白。先前的证据表明，粘附R。立克次体对宿主细胞的作用是由立克次体蛋白介导的。五个单克隆抗体rOmpA，五个rOmpB，和一个立克次体脂多糖（LPS）进行了测试，抑制立克次体附着。抗rOmpA的单克隆抗体均能抑制立克次体对L-929细胞的粘附，抑制率可达90%以上。相反，rOmpB和LPS的单克隆抗体不阻断附着。当使用针对rOmpA和rOmpB的单克隆抗体的Fab片段时，分别观察到与完整单克隆、非粘附和粘附相似的结果。纯化的rOmpA对R.立克次体对宿主细胞的作用。胰蛋白酶能完全消化完整R. rOmpA在立克次体的初始粘附中起重要作用。立克次体对宿主细胞的作用。(C)出版社：Academic Press Limited。
rOmpA and rOmpB are immunodominant, surface-exposed proteins of Rickettsia rickettsii. Prior evidence suggests that adhesion of R. rickettsii to the host cell is mediated by a rickettsial protein. Five monoclonal antibodies to rOmpA, five to rOmpB, and one to the rickettsial lipopolysaccharide (LPS) were tested for inhibition of rickettsial attachment. All the monoclonal antibodies to rOmpA inhibited adhesion of rickettsiae to the L-929 cells with some inhibition rates as high as 90%. In contrast, monoclonal antibodies to rOmpB and LPS did not block attachment. When Fab fragments of monoclonal antibodies against rOmpA and rOmpB were used, similar results were observed as for the intact monoclonals, non-adhesion and adhesion, respectively. Purified rOmpA showed a competitive inhibitive effect on the attachment of R. rickettsii to host cells. Trypsin completely digested rOmpA but not rOmpB from the surface of intact R. rickettsii, resulting in loss of the ability of the rickettsiae to attach to the host cell, rOmpA appears to play an important role in the initial adhesion of R. rickettsii to the host cell. (C) 1998 Academic Press Limited.