Epitope mapping of neutralizing botulinum neurotoxin A antibodies by phage display
Epitope mapping of neutralizing botulinum neurotoxin A antibodies by phage display
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DOI：
10.1128/iai.69.10.6511-6514.2001
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发表时间：
2001-10-01
影响因子：
3.1
通讯作者：
Marks, JD
中科院分区：
文献类型：
--
作者：
Mullaney, BP;Pallavicini, MG;Marks, JD
单链抗体中和活性并结合肉毒杆菌A神经毒素的非重叠表位。利用1.3 kb的结合域cDNA构建了两个噬菌体展示表位文库。针对单链Fv-Fc抗体选择的最小表位对应于氨基酸残基为1115 ~ 1223 （S25）、1131 ~ 1264 （3D12）和889 ~ 1294 （C25）的构象表位。
Single-chain antibodies neutralize activity and bind nonoverlapping epitopes of botulinum A neurotoxin. Two phage display epitope libraries were constructed from the 1.3 kb of binding domain cDNA. The minimal epitopes selected against the single-chain Fv-Fc antibodies correspond to conformational epitopes with amino acid residues 1115 to 1223 (S25), 1131 to 1264 (3D12), and 889 to 1294 (C25).