Application of microchip electrophoresis in the analysis of RNA aptamer-protein interactions
Application of microchip electrophoresis in the analysis of RNA aptamer-protein interactions
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DOI：
10.1080/15257770600683953
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发表时间：
2006-01-01
影响因子：
1.3
通讯作者：
Nishikawa, S
中科院分区：
文献类型：
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作者：
Nishikawa, F;Arakawa, H;Nishikawa, S
DNA和RNA可以通过微芯片电泳（ME）分离，并使用插层荧光染料检测。该方法的优点是传感时间短（< 3分钟），避免了放射性同位素标记检测系统，成本相对较低，并且降低了劳动强度。在本研究中，使用ME分析了RNA适体与蛋白质或肽的相互作用，并检测了随着靶蛋白或肽以剂量依赖性方式增加，与未结合RNA对应的游离适体的回归。我们的结果表明，这种方法适用于简单，快速筛选配体之间的相互作用寡核苷酸和他们的目标。
DNA and RNA can be separated by microchip electrophoresis (ME) and detected using an intercalating fluorescent dye. The advantages of this method are short sensing times (< 3 min), avoidance of a radioisotope labeling detection system, relatively low costs, and reduced labor intensity. In the present study, RNA aptamer-protein or -peptide interactions were analyzed using ME and the regression of free aptamers corresponding to unbound RNA was detected as the target protein or peptide increased in a dose-dependent manner. Our results demonstrate the applicability of this method to simple, rapid ligand screening in the interactions between oligonucleotides and their targets.