Expression of proliferative and preapoptotic molecules in human myometrium and leiomyoma throughout the menstrual cycle
Expression of proliferative and preapoptotic molecules in human myometrium and leiomyoma throughout the menstrual cycle
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DOI：
10.1177/1933719107305866
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发表时间：
2007-10-01
影响因子：
2.9
通讯作者：
Arici, Aydin
中科院分区：
文献类型：
--
作者：
Kayisli, Umit A.;Berkkanoglu, Murat;Arici, Aydin
平滑肌瘤的发病机制可能与性类固醇与旁分泌生长因子相互作用的不平衡有关，旁分泌生长因子可控制有丝分裂和局部免疫的调节。作者研究了可能与此相关的增殖和凋亡前分子的时间和空间表达。参与子宫肌层功能的调节和平滑肌瘤发病机制。采用免疫组化和Western blot分析法研究子宫肌层和平滑肌瘤中Fas配体(FasL)、10号染色体上磷酸酶和张力蛋白同源物缺失(PTEN)以及增殖细胞核抗原(PCNA)的表达，Western blot结果显示，在子宫肌层和平滑肌瘤中，分泌期FasL表达量分别是增殖期的1.8倍和2.3倍。分别（P 分别 = .022 和 .047）。子宫肌层和平滑肌瘤之间的配对比较揭示了平滑肌瘤中较高的 FasL 表达 (P = .003)。相反，与分泌期相比，增殖期的 PCNA 表达在子宫肌层和平滑肌瘤中分别高 4.6 倍和 3.7 倍（分别为 P = 0.041 和 0.034）。子宫肌层和平滑肌瘤之间的配对比较揭示了平滑肌瘤中 PCNA 的表达较高。此外，与子宫肌层相比，在平滑肌瘤中检测到较低的 PTEN 表达 (P < .032)。免疫组化结果显示FasL、PTEN和PCNA在子宫肌层和平滑肌瘤中表达，与Western blot分析结果一致。结果提示FasL、PTEN和PCNA可能参与平滑肌瘤的病理生理学。平滑肌瘤中较高的FasL水平可能与通过诱导免疫细胞凋亡来抑制局部免疫有关，而较高水平的PCNA和较低水平的PTEN可能与平滑肌瘤中有丝分裂增加和细胞凋亡减少有关。
The pathogenesis of leiomyoma may be related to an imbalance in. the interaction of sex steroids with paracrine growth factors that may control the modulation of mitogenesis and local immunity. The authors investigate the temporal and spatial expression of proliferative and preapoptotic molecules that may par. ticipate in the modulation of myometrial function and leiomyoma pathogenesis. Immunohistochemistry and Western blot analysis are used to investigate Fas ligand (FasL), phosphatase and tensin homolog deletion on chromosome 10 (PTEN), and proliferating cell nuclear antigen (PCNA) expression in myometrium and leiomyoma, Western blot results show that in the secretory phase, FasL expression, is 1.8-fold and 2.3-fold higher compared with the proliferative phase in the myometrium and leiomyoma, respectively (P = .022 and .047, respectively). A paired comparison between myometrium and leiomyoma reveals higher FasL expression in the leiomyoma (P = .003). On the contrary, when compared with the secretory phase, PCNA expression during the proliferative phase is 4.6-fold and 3.7-fold higher in the myometrium and leiomyoma, respectively (P = .041 and .034, respectively). A paired comparison between myometrium and leiomyoma reveals higher PCNA expression in the leiomyoma. Furthermore, lower PTEN expression is detected in the leiomyoma compared with the myometrium (P < .032). Immunohistochemistry results reveal that FasL, PTEN, and PCNA arc expressed in the myometrium and leiomyoma, consistent with the results from the Western blot analysis. The results suggest that FasL, PTEN, and PCNA may be involved in the pathophysiology of leiomyoma. A higher FasL level in the leiomyoma is likely to correspond to suppression of local immunity by inducing apoptosis of immune cells, while a higher level of PCNA and a lower level of PTEN may be related to increased mitogenesis and decreased apoptosis in leiomyoma.