Chondrogenic differentiation of human mesenchymal stem cells from umbilical cord blood in chemically synthesized thermoreversible polymer.
Chondrogenic differentiation of human mesenchymal stem cells from umbilical cord blood in chemically synthesized thermoreversible polymer.
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发表时间：
2008-08
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通讯作者：
I-Ting Kao;Chao-Ling Yao;Yu-Jen Chang;T. Hsieh;Shiaw-Min Hwang
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作者：
I-Ting Kao;Chao-Ling Yao;Yu-Jen Chang;T. Hsieh;Shiaw-Min Hwang
支架为细胞在三维环境中的分布、生长、分化和细胞外基质积累提供了模板。最近的研究表明，在体外和体内研究中，支架具有促进关节软骨修复的潜力。来源于人脐带血的间充质干细胞（CBMSCs）的特征在于其分化为间充质谱系细胞类型（包括软骨细胞、成骨细胞和脂肪细胞）的多能性。在这项研究中，CBMSCs的软骨形成进行了化学合成的热可逆凝胶聚合物（TGP）。将CBMSCs包埋在TGP中，并补充抗坏血酸和转化生长因子β 3。诱导4周后，结果显示CBMSCs形成球状颗粒，并且尺寸增大。在TGP中诱导的细胞表达软骨细胞的II型胶原、聚集蛋白聚糖和Sox 9的特异性mRNA。TGP包埋的CBMSCs的糖胺聚糖分泌量与DNA含量的比值明显高于传统的诱导方法。结果表明，化学合成的TGP为CBMSCs向软骨细胞分化提供了良好的三维培养环境，有望在临床上应用于诱导CBMSCs向软骨细胞分化，用于软骨修复。
Scaffolds provide a template for cell distribution, growth, differentiation and extracellular matrix accumulation in a three-dimensional environment. Recent studies have demonstrated the potential of scaffolds for enhancing articular cartilage repair both in vitro and in vivo investigations. Mesenchymal stem cells derived from human umbilical cord blood (CBMSCs) have been characterized by their multipotency to differentiate into mesenchyme-lineage cell types, including chondrocytes, osteoblasts, and adipocytes. In this study, chondrogenesis of CBMSCs was performed in a chemically synthesized thermoreversible gelation polymer (TGP). CBMSCs were embedded in the TGP and supplemented with ascorbic acid and transforming growth factor-beta 3. After a 4-week induction, the results showed that CBMSCs formed into spheroid pellets and increased in size. The induced cells in the TGP expressed specific mRNA of collagen type II, aggrecan, and Sox9 for chondrocytes. Furthermore, CBMSCs embedded in TGP had higher ratio of glycosaminoglycan secretion to DNA content than the traditional induction method by aggregating pellet culture. These results demonstrated that chemically synthesized TGP provided a competent 3-dimentional culture environment for CBMSCs to differentiate into chondrocytes and may be applied clinically to induce chondrogenic differentiation of CBMSCs for cartilage repair in the future.