Molecular biomimetics: Utilizing naturels molecular ways in practical engineering
Molecular biomimetics: Utilizing naturels molecular ways in practical engineering
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DOI：
10.1016/j.actbio.2006.10.009
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发表时间：
2007-05-01
影响因子：
9.7
通讯作者：
Sarikaya, Mehmet
中科院分区：
文献类型：
--
作者：
Tamerler, Candan;Sarikaya, Mehmet
在自然界中，蛋白质是通过其在从单细胞到多细胞生物体的生物系统中的特定识别和相互作用来完成许多功能的机器。生物分子-材料相互作用是通过分子特异性来完成的，从而导致在维度层次的所有尺度上形成受控结构和功能。通过进化、分子识别以及通过连续的突变和选择循环而发展的功能。以生物学为指导，我们现在可以理解、设计和控制肽-材料相互作用，并利用它们为实际应用定制新型材料和系统。我们采用组合生物学方案来展示肽库，无论是在细胞表面上油还是在噬菌体上，以选择特定于各种实用材料系统的短肽。在选择步骤之后，我们通过实验确定了肽结合的动力学和稳定性，以通过建模了解结合的肽结构并通过原子力显微镜了解其组装。这些肽经过进一步改造，具有多个重复序列，或者改变它们的氨基酸序列，以适应它们的功能。纳米颗粒和含有纳米和微米尺度多材料图案的平面无机基材均用于分子结构的自定向固定。分子仿生方法为多功能分子系统的设计和利用开辟了新途径，其应用范围广泛，从组织工程、药物输送和生物传感器到纳米技术和生物修复。在这里，我们给出了生物学、肽选择和工程中与无机物亲和力的蛋白质介导的功能材料的例子，展示了在材料科学、工程和医学中的潜在用途。并描述未来的前景。 (C)) 2006 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
In nature, proteins are the machinery that accomplish many functions through their specific recognition and interactions ill biological systems from single-celled to multicellular organisms. Biomolecule-material interaction is accomplished via molecular specificity, leading to the formation of controlled structures and functions at all scales of dimensional hierarchy. Through evolution, molecular recognition and, consequently, functions developed through successive cycles of mutation and selection. Using biology as a guide, we can now understand, engineer and control peptide-material interactions and exploit these to tailor novel materials and systems for practical applications. We adapted combinatorial biology protocols to display peptide libraries, either oil the cell surface or on phages, to select short peptides specific to a variety of practical materials systems. Following the selection step, we determined the kinetics and stability of peptide binding experimentally to understand the bound peptide structure via modeling and its assembly via atomic force microscopy. The peptides were further engineered to have multiple repeats or their amino acid sequences varied to tailor their function. Both nanoparticles and flat inorganic substrates containing multimaterials patterned at the nano- and microscales were used for self-directed immobilization of molecular constructs. The molecular biomimetic approach opens up new avenues for the design and utilization of multifunctional molecular systems with wide ranging applications, from tissue engineering, drug delivery and biosensors, to nanotechnology and bioremediation. Here we give examples of protein-mediated functional materials in biology, peptide selection and engineering with affinity to inorganics, demonstrate potential utilizations in materials science, engineering and medicine. and describe future prospects. (C)) 2006 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.