Clinical peptidomic analysis by a one‐step direct transfer technology: Its potential utility for monitoring of pathophysiological status in female reproductive system disorders
Clinical peptidomic analysis by a one‐step direct transfer technology: Its potential utility for monitoring of pathophysiological status in female reproductive system disorders
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DOI：
10.1111/jog.12140
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发表时间：
2013-10
影响因子：
1.6
通讯作者：
Y. Araki;D. Nonaka;Kensuke Hamamura;M. Yanagida;H. Ishikawa;M. Banzai;M. Maruyama;Shuichiro Endo;A. Tajima;Lyang-ja Lee;M. Nojima;K. Takamori;Koyo Yoshida;S. Takeda;Kenji Tanaka
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文献类型：
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作者：
Y. Araki;D. Nonaka;Kensuke Hamamura;M. Yanagida;H. Ishikawa;M. Banzai;M. Maruyama;Shuichiro Endo;A. Tajima;Lyang-ja Lee;M. Nojima;K. Takamori;Koyo Yoshida;S. Takeda;Kenji Tanaka
迄今为止，许多研究都在寻找候选分子或临床检查方法，作为监测顽固性疾病（如癌症）的潜在生物标志物。过去十年积累的证据表明，许多蛋白水解肽出现在人体体液中，包括外周血，与个体的健康状况有关。虽然使用质谱法对整个肽（“肽穹”）进行分析被认为是最强大和最有前途的实验方法之一，但由于方法学的限制，它未能识别临床血液样本中的生物标志物。一般来说，用于血液蛋白质组学分析的常用技术需要在质谱分析之前去除大量的血清/血浆蛋白质，这一步骤似乎导致在分析过程中忽略了重要的生物标志物。在这里，我们简要介绍了一种新的定量肽组学分析方法，该方法采用一步直接转移技术，无需耗尽主要血液蛋白。利用该技术，我们报告了妊娠高血压患者血清肽组学分析作为临床模型的实验数据。此外，我们还提到了这种方法在监测女性生殖系统疾病的病理生理状态方面的潜在效用。
To date, numerous studies have searched for candidate molecules or clinical examination methods as potential biomarkers for monitoring intractable diseases, such as carcinomas. Evidence accumulated over the past decade shows that many proteolytic peptides appear in human humoral fluids, including peripheral blood, in association with an individual's health condition. Although an analysis of the whole peptide (the ‘peptidome’) using mass spectrometry is thought to be one of the most powerful and promising experimental approaches, it has failed to identify biomarkers in the clinical blood samples, presumably due to the methodological limitations. In general, commonly used techniques for proteomic analysis of blood require the removal of large amounts of serum/plasma proteins prior to mass spectrometry analysis, and this step seems to have resulted in the overlooking of important biomarkers during the analytical process. Here, we provide a brief overview of a new quantitative peptidomic analysis by a one‐step direct transfer technology without depletion of major blood proteins. Using this technology, we herein report experimental data on serum peptidomic analysis for patients with pregnancy‐induced hypertension as a clinical model. In addition, we refer to the potential utility of this approach for the monitoring of pathophysiological status in female reproductive system disorders in general.