Maternal sleep deprivation at different stages of pregnancy impairs the emotional and cognitive functions, and suppresses hippocampal long-term potentiation in the offspring rats.
Maternal sleep deprivation at different stages of pregnancy impairs the emotional and cognitive functions, and suppresses hippocampal long-term potentiation in the offspring rats.
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母亲在妊娠不同阶段的睡眠剥夺会损害子代大鼠的情绪和认知功能，并抑制海马长时程增强。
DOI：
10.1186/s13041-016-0197-3
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发表时间：
2016-02-15
期刊：
影响因子：
3.6
通讯作者：
Han H
中科院分区：
文献类型：
--
作者：
Peng Y;Wang W;Tan T;He W;Dong Z;Wang YT;Han H
怀孕期间睡眠不足是一个严重的公共卫生问题，因为它会影响孕妇和新生儿的健康。然而，在怀孕的不同阶段睡眠剥夺是否对后代的情绪和认知功能有相似的影响还没有得到很好的研究，如果有的话，潜在的细胞机制也仍然知之甚少。方法在妊娠早期（妊娠第1 ~ 7天）、中期（妊娠第8 ~ 14天）和晚期（妊娠第15 ~ 21天），采用轻柔处理的方法，每天剥夺大鼠睡眠6 h。对子代大鼠（出生后42 ~ 56天）的情绪和认知功能以及海马长期增强（LTP）功能进行了测试。结果后代表现出海马依赖性空间学习和记忆受损，抑郁和焦虑样行为增加。brdu阳性细胞的定量显示，与对照组相比，成年海马神经发生明显减少。电生理记录显示，母亲睡眠剥夺损害了海马CA1 LTP，减少了基础突触传递，反映在海马CA1锥体神经元的微型兴奋性突触后电流的频率和幅度下降。综上所述，这些结果表明，在怀孕的不同阶段，母亲的睡眠剥夺会破坏后代的情绪和认知功能，这可能是由于海马LTP和基础突触传递的抑制。
BackgroundSleep deprivation during pregnancy is a serious public health problem as it can affect the health of pregnant women and newborns. However, it is not well studied whether sleep deprivation at different stages of pregnancy has similar effects on emotional and cognitive functions of the offspring, and if so, the potential cellular mechanisms also remain poorly understood.MethodsIn the present study, the pregnant rats were subjected to sleep deprivation for 6 h per day by gentle handling during the first (gestational days 1–7), second (gestational days 8–14) and third trimester (gestational days 15–21) of pregnancy, respectively. The emotional and cognitive functions as well as hippocampal long-term potentiation (LTP) were tested in the offspring rats (postnatal days 42-56).ResultsThe offspring displayed impaired hippocampal-dependent spatial learning and memory, and increased depressive- and anxiety-like behaviors. Quantification of BrdU-positive cells revealed that adult hippocampal neurogenesis was significantly reduced compared to control. Electrophysiological recording showed that maternal sleep deprivation impaired hippocampal CA1 LTP and reduced basal synaptic transmission, as reflected by a decrease in the frequency and amplitude of miniature excitatory postsynaptic current in the hippocampal CA1 pyramidal neurons.ConclusionsTaken together, these results suggest that maternal sleep deprivation at different stages of pregnancy disrupts the emotional and cognitive functions of the offspring that might be attributable to the suppression of hippocampal LTP and basal synaptic transmission.