Phylogenetic analysis of invertebrate lysozymes and the evolution of lysozyme function
Phylogenetic analysis of invertebrate lysozymes and the evolution of lysozyme function
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DOI：
10.1007/s00239-001-0061-6
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发表时间：
2002-05-01
影响因子：
3.9
通讯作者：
Deutsch, JS
中科院分区：
文献类型：
--
作者：
Bachali, S;Jager, M;Deutsch, JS
我们分离并测序了编码六种双壳贝类溶菌酶的cDNA。比对和系统发育分析表明，它们与最近描述的双壳类溶菌酶、水蛭去稳定酶以及大量基因组和cDNA分析中推定的蛋白质一起，属于无脊椎动物类型的溶菌酶（i型），由Jolles和Jolles首次描述（1975）。我们确定了贻贝溶菌酶基因的基因组结构。我们提供的证据表明，该基因的中心外显子是同源的鸡溶菌酶基因的第二外显子，属于C型。我们建议，这一领域的起源可以追溯到进化的起源两侧对称的动物。系统发育分析表明，i型蛋白形成一个单系家族。
We isolated and sequenced the cDNAs coding for lysozymes of six bivalve species. Alignment and phylogenetic analysis showed that, together with recently described bivalve lysozymes, the leech destabilase, and a number of putative proteins from extensive genomic and cDNA analyses, they belong to the invertebrate type of lysozymes (i type), first described by Jolles and Jolles (1975). We determined the genomic structure of the gene encoding the lysozyme of Mytilus edulis, the common mussel. We provide evidence that the central exon of this gene is homologous to the second exon of the chicken lysozyme gene, belonging to the c type. We propose that the origin of this domain can be traced back in evolution to the origin of bilaterian animals. Phylogenetic analysis suggests that i-type proteins form a monophyletic family.