A New Protein Factor in the Product Formation of Non-Reducing Fungal Polyketide Synthase with a C-Terminus Reductive Domain
A New Protein Factor in the Product Formation of Non-Reducing Fungal Polyketide Synthase with a C-Terminus Reductive Domain
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DOI：
10.4014/jmb.1504.04086
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发表时间：
2015-10-01
影响因子：
2.8
通讯作者：
Kwon, Hyung-Jin
中科院分区：
文献类型：
--
作者：
Balakrishnan, Bijinu;Chandran, Ramya;Kwon, Hyung-Jin
通过具有C-末端还原结构域（-R）的迭代非还原真菌聚酮酶（NR-fPKS）合成氮杂菲酮聚酮。几个azaphilone生物合成基因簇含有一个假定的丝氨酸水解酶基因; Monopolia azaphilone色素（MAzP）基因簇含有mppD。通过mppD的敲除，MAzP生产率显著降低，并且MAzP NR-fPKS-R基因（MpPKS 5）仅当与mppD共表达时才在酵母中产生其产物。保守的Ser/Asp/His残基的mppD的定点突变消除了来自MpPKS 5/mppD共表达的产物形成。因此，MppD及其同源物被认为是参与NR-fPKS-R产物形成的新蛋白因子。
Azaphilone polyketides are synthesized by iterative non-reducing fungal polyketide synthases (NR-fPKSs) with a C-terminus reductive domain (-R). Several azaphilone biosynthetic gene clusters contain a putative serine hydrolase gene; the Monascus azaphilone pigment (MAzP) gene cluster harbors mppD. The MAzP productivity was significantly reduced by a knockout of mppD, and the MAzP NR-fPKS-R gene (MpPKS5) generated its product in yeast only when co-expressed with mppD. Site-directed mutations of mppD for conserved Ser/Asp/His residues abolished the product formation from the MpPKS5/mppD co-expression. MppD and its homologs are thus proposed as a new protein factor involved in the product formation of NR-fPKS-R.