Identification of carbohydrate structures that are possible receptors for Neisseria gonorrhoeae.
Identification of carbohydrate structures that are possible receptors for Neisseria gonorrhoeae.
复制标题
鉴定可能是淋病奈瑟菌受体的碳水化合物结构。
DOI：
10.1073/pnas.85.13.4902
复制
发表时间：
1988
影响因子：
11.1
通讯作者：
Karlsson,KA
中科院分区：
文献类型：
--
作者：
Stromberg,N;Deal,C;Nyberg,G;Normark,S;So,M;Karlsson,KA
测定淋球菌的不同菌株和同基因变体结合从各种来源提取的糖脂的能力。在大量参考糖脂中，仅观察到与乳糖神经酰胺的结合[Gal（β 1 - 4）葡萄糖（β 1 - 1）Cer]、异葡萄糖三己糖神经酰胺[Gal（α 1 - 3）半乳糖（β 1 - 4）葡萄糖（β 1 - 1）Cer]、神经节三己糖神经酰胺[GalNAc（β 1 - 4）Gal（β 1 - 4）Glc（β 1 - 1）Cer]和神经节四糖神经酰胺[Gal（β 1 - 3）GalNAc（β 1 - 4）Gal（β 1 - 4）Glc（β 1 - 1）Cer]。后两种糖脂以最高的亲和力结合淋球菌。乳糖神经酰胺和神经节三己糖神经酰胺在ME180细胞的糖脂制剂中被发现，ME180细胞是一种源自人宫颈癌的上皮细胞系，因此可能是淋球菌的受体。结合上述糖脂的淋球菌表面组分是不同于菌毛蛋白和蛋白II的蛋白质。
Different strains and isogenic variants of Neisseria gonorrhoeae were assayed for their ability to bind glycolipids extracted from various sources. Among a large number of reference glycolipids, binding was observed only to lactosylceramide [Gal(beta 1-4)Glc(beta 1-1)Cer], isoglobotriaosylceramide [Gal(alpha 1-3)Gal(beta 1-4)Glc(beta 1-1)Cer], gangliotriaosylceramide [GalNAc(beta 1-4)Gal(beta 1-4)Glc(beta 1-1)Cer], and gangliotetraosylceramide [Gal(beta 1-3)GalNAc(beta 1-4)Gal(beta 1-4)Glc(beta 1-1)Cer]. The latter two glycolipids bound gonococci with the highest affinity. Lactosylceramide and gangliotriaosylceramide were found in glycolipid preparations from ME180 cells, an epithelial cell line derived from a human cervical carcinoma, and thus are possible receptors for gonococci. The gonococcal surface component that bound the above glycolipids is a protein distinct from pilin and protein II.