Charcot-Leyden crystal protein and eosinophil granule major basic protein in sputum of patients with respiratory diseases.
Charcot-Leyden crystal protein and eosinophil granule major basic protein in sputum of patients with respiratory diseases.
复制标题
DOI：
10.1164/arrd.1984.130.6.1072
复制
发表时间：
1984-12
期刊：
影响因子：
--
通讯作者：
Philippe J. Dor;Steven J. Ackerman;G. Gleich
中科院分区：
文献类型：
--
作者：
Philippe J. Dor;Steven J. Ackerman;G. Gleich
痰嗜酸性粒细胞增多是支气管哮喘的特征，哮喘患者的痰标本中含有嗜酸性粒细胞来源的物质，包括Charcot-Leyden晶体（CLC）和嗜酸性粒细胞颗粒主要碱性蛋白（MBP）。先前的观察表明，痰中MBP水平升高与哮喘有关。为了确定哮喘患者中CLC蛋白是否也升高，我们用特异性放射免疫法测定了106例连续呼吸系统疾病患者和10例哮喘住院患者的痰标本中MBP和CLC蛋白的含量。所有痰液样本均检测到CLC蛋白，116个样本中CLC蛋白水平平均为3.5微克/毫升（范围为0.01 ~ 25微克/毫升）。急性哮喘患者和某些其他呼吸系统疾病患者的痰标本中，当与支气管肺感染相关时，CLC蛋白显著升高。相比之下，116个样本中的MBP水平平均为0.32微克/毫升（范围为0.01至8.8微克/毫升），并且仅在无症状或急性哮喘患者中显著升高。支气管肺感染患者的痰MBP没有升高，除非同时存在急性哮喘。因此，痰中MBP水平升高与哮喘特异性相关，而在某些患者组中，CLC蛋白水平升高与哮喘和支气管肺感染均相关。
Sputum eosinophilia is characteristic of bronchial asthma, and sputum specimens from patients with asthma contain eosinophil-derived substances including Charcot-Leyden crystals (CLC) and the eosinophil granule major basic protein (MBP). Prior observations have indicated that an elevated sputum level of MBP is associated with asthma. To determine whether CLC protein was also elevated in asthma, we measured by specific radioimmunoassays the quantities of MBP and CLC protein in sputum specimens from 106 consecutive patients with various respiratory diseases and in sputum specimens from 10 patients hospitalized for asthma. The CLC protein was detected in all sputum samples, and the levels in the 116 samples averaged 3.5 micrograms/ml (range, 0.01 to 25 micrograms/ml). The CLC protein was significantly elevated in sputum specimens from patients with acute asthma and from patients with certain other respiratory diseases when associated with bronchopulmonary infection. In contrast, MBP levels in the 116 samples averaged 0.32 micrograms/ml (range, 0.01 to 8.8 micrograms/ml) and were significantly elevated only in patients with asymptomatic or acute asthma. Sputum MBP was not elevated in patients with bronchopulmonary infections unless acute asthma was also present. Thus, an elevated sputum MBP level was specifically associated with asthma, whereas an elevated CLC protein level was associated with both asthma and with bronchopulmonary infection in certain patient groups.