Salivary soluble CD44: A potential molecular marker for head and neck cancer
Salivary soluble CD44: A potential molecular marker for head and neck cancer
复制标题
DOI：
10.1158/1055-9965.epi-04-0546
复制
发表时间：
2005-03-01
影响因子：
3.8
通讯作者：
Goodwin, WJ
中科院分区：
文献类型：
--
作者：
Franzmann, EJ;Reategui, EP;Goodwin, WJ
目的：头颈部鳞状细胞癌（HNSCC）是一种使人衰弱的疾病，治愈率仅为50%。如果早期诊断，存活率可以达到80%，但目前还没有实用的早期检测方法。CD 44包含一个同种型家族，在某些肿瘤中，其在组织和循环中选择性剪接和过表达。在这里，我们研究唾液可溶性CD 44（solCD 44）的表达在HNSCC患者和正常对照，以确定其潜在的作为一个screeningtool.Method：我们做了solCD 44 ELISA唾液从26 HNSCC患者，10名正常志愿者，条件培养基（CM）的4 HNSCC细胞系，和1 CD 44-阴性细胞系（COS-7）。用Western blot法检测2株HNSCC细胞系（UMSS 11B和FaDu）、COS-7、3株HNSCC和2份正常唾液标本的CM，以验证ELISA抗体的特异性。与正常对照组相比，HNSCC患者的SolCD 44水平显著升高（HNSCC患者为7.85 ng/mL，正常对照组为1.09 ng/mL，P < 0.001）。SolCD 44水平与肿瘤大小、分期、复发、放射治疗史或烟草和酒精风险因素无显著差异。一个65至75 kDa的带，对应于solCD 44，在所有的HNSCC细胞系CM和唾液中检测到，而正常样品显示一个较暗的带或undetected.Conclusion：在这个初步分析中，唾液solCD 44 ELISA似乎有效地检测HNSCC在所有阶段。需要进一步研究，因为早期发现在这种疾病中显然很重要。
Objective: Head and neck squamous cell carcinoma (HNSCC) is a debilitating disease which is cured only 50% of the time. If diagnosed early, survival rates could reach 80%, but there is currently no practical method for early detection. CD44 comprises a family of isoforms that, in certain tumors, are alternatively spliced and overexpressed in tissues and circulation. Here we examine salivary soluble CD44 (solCD44) expression in HNSCC patients and normal controls to determine its potential as a screening tool.Method: We did a solCD44 ELISA on saliva from 26 HNSCC patients, 10 normal volunteers, conditioned media (CM) of 4 HNSCC cell lines, and 1 CD44-negative cell line (COS-7). Western blot was done on CM from 2 HNSCC cell lines (UMSS11B and FaDu), COS-7,3 HNSCC, and 2 normal saliva specimens to verify ELISA antibody specificity. SolCD44 levels were significantly elevated in HNSCC patients compared with normal controls (7.85 ng/mL for HNSCC patients and 1.09 ng/mL for normal controls, P < 0.001).Results: The test detected 79% of mucosally invasive HNSCC using preliminary cutoff points. SolCD44 levels did not vary significantly with tumor size, stage, recurrence, history of radiation treatment, or tobacco and alcohol risk factors. A 65 to 75 kDa band, corresponding to solCD44, was detected in all of the HNSCC cell line CM and saliva whereas normal samples showed a fainter band or were undetectable.Conclusion: In this preliminary analysis, the salivary solCD44 ELISA seems to effectively detect HNSCC at all stages. Further study is indicated because early detection is clearly important in this disease.