Three-dimensional nanofibrous microenvironment designed for the regulation of mesenchymal stem cells
Three-dimensional nanofibrous microenvironment designed for the regulation of mesenchymal stem cells
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专为间充质干细胞调控而设计的三维纳米纤维微环境
DOI：
10.1007/s13204-018-0877-7
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发表时间：
2018-11-01
影响因子：
--
通讯作者：
Gao, Yanzheng
中科院分区：
文献类型：
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作者：
Jin, Lin;Zhang, Xingcai;Gao, Yanzheng
三维功能性纳米纤维微环境在细胞生长调控中起着关键作用，因为它可以模拟天然细胞外基质结构。三维功能纳米纤维作为一种重要的纳米纤维支架，在组织工程、药物释放、生物传感器等生物医学领域显示出广阔的应用前景。近年来，这些基于生物相容性天然材料的功能性纳米纤维支架具有良好的柔韧性和生物化学特性，在组织工程应用中显示出巨大的潜力。本文采用静电纺丝和化学接枝相结合的方法制备了三维硫酸软骨素表面修饰的丝素纳米纤维。所制备的3D CS-SNFs具有良好的生物相容性。此外，有许多深度互连的孔（大于20 μm），用于增强细胞进入CS-SNF和体外3D细胞培养。三维CS-SNF有利于细胞粘附、营养输送和排泄物的排出，这为那些渗透到支架内部的细胞提供了健康的生长环境和生活条件。我们的研究结果表明，三维CS-SNFs显示更高程度的促进骨髓间充质干细胞的粘附和成骨分化，与三维生丝纳米纤维相比。这些结果表明，所制备的3D CS-SNFs可以为细胞生长、粘附和优异的成骨分化提供合适的3D微环境。这项工作为3D细胞培养构建损伤骨修复和再生铺平了新的道路。
The three-dimensional functional nanofibrous microenvironment plays a key role in the regulation of cellular growth, because it can mimic the natural extracellular matrix structure. As an important nanofibrous scaffold, 3D functional nanofibers display broad application prospects in biomedical areas, such as tissue engineering, drug release, and biosensors. Recently, these functional nanofibrous scaffolds based on biocompatible natural materials with excellent flexibility and biochemical characteristics show great potential in tissue engineering applications. Herein, we prepared 3D chondroitin sulfate surface-modified silk nanofibers (3D CS-SNFs) using a combination of electrospinning and chemical grafting methods. The as-prepared 3D CS-SNFs exhibited excellent biocompatible properties. Moreover, there are numerous deeply interconnected pores (larger than 20 µm) for enhanced cellular entry into CS-SNFs and for 3D cell culture in vitro. The 3D CS-SNFs are beneficial to cell adhesion, nutrient delivery, and excretion of excrement, which provides healthy growth environment and living conditions for those cells that penetrate into the interior of the scaffold. Our results indicate that the 3D CS-SNFs show much higher degrees of promotion of bone marrow mesenchymal stem cells adhesion and osteogenic differentiation, as compared with the 3D raw silk nanofibers. These results demonstrate that the as-prepared 3D CS-SNFs could provide a suitable 3D microenvironment for cellular growth, adhesion, and excellent osteogenic differentiation. This work paves a new way for 3D cell culture construction for damaged bone repair and regeneration.