ADHESIVE SUBSTRATES FOR FIBRONECTIN
ADHESIVE SUBSTRATES FOR FIBRONECTIN
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DOI：
10.1002/jcp.1041090314
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发表时间：
1981-01-01
影响因子：
5.6
通讯作者：
SCHOEN, RC
中科院分区：
文献类型：
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作者：
KLEBE, RJ;BENTLEY, KL;SCHOEN, RC
为了促进细胞粘附，纤连蛋白必须首先被合适的底物激活。对材料（52）结合纤连蛋白、激活纤连蛋白的细胞粘附性质和支持细胞生长的能力进行了调查。许多塑料、多糖、金属和陶瓷支持细胞生长以及纤维连接蛋白依赖的细胞附着。鉴定了几种其他底物，它们在促进细胞附着或生长方面无活性。疏水性底物在纤连蛋白活化中是活性的;亲水性底物是无活性的。由于纤连蛋白与化学组成极其不同的基质结合，因此纤连蛋白与这种基质的结合本质上是非特异性的。由于蛋白质预处理的所有基质，除了胶原蛋白和聚（L-赖氨酸），取消了纤连蛋白的物理结合，纤连蛋白的人工基质的结合可能是归因于一个非特异性的疏水蛋白基质相互作用。纤维连接蛋白与胶原的相互作用可能具有生物学特异性。聚（甲基丙烯酸羟乙酯）（聚（HEMA）），这是以前被证明是不粘附的细胞，在这里被证明是独特的，它不能结合纤连蛋白。向聚（HEMA）中加入1 ppm的粘合剂聚合物可使纤连蛋白结合发生。
In order to promote cell attachment, fibronectin must first undergo activaton by a suitable substrate. Materials (52) were surveyed for their ability to bind fibronectin, to activate the cell-adhesive property of fibronectin and to support the growth of cells. Many plastics, polysaccharides, metals and ceramics supported cell growth as well as the fibronectin-dependent attachment of cells. Several other substrates were identified that were inactive in promoting either cell attachment or growth. Hydrophobic substrates were active in fibronectin activation; hydrophilic substrates were inactive. Since fibronectin binds to substrata of extremely varied chemical composition, the binding of fibronectin to such substrata is nonspecific in nature. Since protein pretreatment of all substrata, except collagen and poly(L-lysine), abolished the physical binding of fibronectin, the binding of fibronectin to artificial substrata is probably ascribable to a nonspecific hydrophobic protein-substratum interaction. The interaction between fibronectin and collagen may display biological specificity. Poly(hydroxyethylmethacrylate)(poly(HEMA)), which was previously shown to be nonadhesive for cells, is demonstrated here to be unique in its inability to bind fibronectin. Addition of 1 part/million of an adhesive polymer to poly(HEMA) permits fibronectin binding to occur.