Molecular analysis of Streptococcus pyogenes strains isolated from Chinese children with pharyngitis
Molecular analysis of Streptococcus pyogenes strains isolated from Chinese children with pharyngitis
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DOI：
10.1016/j.diagmicrobio.2010.09.011
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发表时间：
2011-02-01
影响因子：
2.9
通讯作者：
Yang, Yonghong
中科院分区：
文献类型：
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作者：
Chang, Hesheng;Shen, Xuzhuang;Yang, Yonghong
化脓性链球菌是一种重要的革兰氏阳性细菌病原体，可引起人类多种疾病，其中以链球菌性咽炎最为常见。在这项工作中，共185 S。采用超抗原（SAg）基因分型、elm基因分型和脉冲场凝胶电泳（PFGE）等方法对中国儿童咽炎化脓性链球菌进行了分析。58株（31.4%）分离株还进行了多位点序列分型（MLST）。结果表明，emm1菌株中speA和speJ基因的携带率分别为88.5%和83.6%，speI基因的携带率仅为13.1%。与此相反，平静的12型菌株没有一个具有speJ，少数菌株具有speA（5.2%），大多数菌株具有speI（91.7%）。PFGE分析显示了25个不同的聚类，并对2个优势emm型菌株进行了MLST，emm12菌株属于ST 36，而emm1菌株属于ST 28。就该群体而言，emm1和emm12是S.我国儿童咽炎相关的化脓菌菌株。大多数咽炎菌株可以被26价疫苗覆盖。一个强的对应关系，发现只有在emm型的方向上的SAg配置文件和PFGE类型，但不是在相反的方向。(C)2011 Elsevier Inc. All rights reserved.
Streptococcus pyogenes is an important gram-positive bacterial pathogen that causes various human diseases, of which streptococcal pharyngitis is the most common. In this work, a total of 185 S. pyogenes isolated from Chinese children with pharyngitis was analyzed by superantigen (SAg) genes, elm genotyping, and pulsed-field gel electrophoresis (PFGE). Fifty-eight (31.4%) isolates were also typed by rnultilocus sequence typing (MLST). The results indicate that most of the emm1 isolates possessed speA (88.5%) and speJ (83.6%), and few isolates possessed speI gene (13.1%). In contrast, none of the calm 12-type isolates possessed speJ; few isolates possessed speA (5.2%); and most of the isolates possessed speI (91.7%). PFGE analysis revealed 25 different clusters, and MLST was performed for 2 predominant emm-type isolates; emm 12 isolates belonged to ST36 while emm1 isolates belonged to ST28. As far as this collection is concerned, emm1 and emm12 are the prevalent genotypes among S. pyogenes strains associated with children's pharyngitis in China. Most of the pharyngitis strains can be covered by a 26-valent vaccine. A strong correspondence is found only in the direction of emm type for both SAg profiles and PFGE types but not in the reverse direction. (C) 2011 Elsevier Inc. All rights reserved.