SEX-PHEROMONE BLEND OF MANDUCA-SEXTA - RESPONSES OF CENTRAL OLFACTORY INTERNEURONS TO ANTENNAL STIMULATION IN MALE MOTHS
SEX-PHEROMONE BLEND OF MANDUCA-SEXTA - RESPONSES OF CENTRAL OLFACTORY INTERNEURONS TO ANTENNAL STIMULATION IN MALE MOTHS
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DOI：
10.1002/arch.940100404
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发表时间：
1989-01-01
影响因子：
2.2
通讯作者：
DOOLITTLE, RE
中科院分区：
文献类型：
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作者：
CHRISTENSEN, TA;HILDEBRAND, JG;DOOLITTLE, RE
最近，对雌蛾信息素腺体外表面的溶剂漂洗进行了化学分析，发现有12种醛的混合物，其中包括先前已知为性信息素成分的(E，Z)-10，12-十六碳二烯(Bombykal)。以往的电生理学研究表明，雄性触角叶中的嗅觉(中脑)中间神经元对同侧触角的信息素刺激表现出广泛的反应。这些实验是用信息素腺体的粗提物和两种合成化合物进行的：主要信息素成分家蚕和(E，Z)-11，13-十五碳二烯，这是雌性“S”信息素混合物中第二种成分的模拟物。使用细胞内的方法，我们现在重新检查了类似的嗅觉中间神经元，使用了12种化学识别成分中的每一种，以及它们的各种组合的合成混合物。从女性腺体中分离出的12种成分中，有8种在雄性嗅觉间神经元中引发了某种形式的反应。根据生化和行为学数据，最有效的是蚕豆和两个三烯，(E，E，E)-和(E，E，Z)-10，12，14-十六碳三烯。我们还得出结论，C15二烯醛对天线上的一种三烯醛受体具有选择性，但远不如天然的三烯醛刺激剂有效。
Recently, chemical analysis of solvent rinses of the external surfaces of pheromone glands from female Manduca sexta revealed a blend of 12 aldehydes, including the previously identified sex pheromone component, (E,Z)-10,12-hexadecadienal (bombykal). Previous electrophysiological studies showed that olfactory (deutocerebral) interneurons in the antennal lobes of males exhibited a wide range of responsiveness to pheromonal stimulation of the ipsilateral antenna. These experiments were performed with crude extracts of pheromone glands as well as two synthetic compounds: the major pheromone component, bombykal, and (E,Z)-11,13-pentadecadienal, a mimic of a second component of the female''s pheromone blend. Using intracellular methods, we have now reexamined similar olfactory interneurons, using each of the 12 chemically identified components as well as synthetic blends of various combinations of them. Eight of the 12 components isolated from female glands elicited some form of response in olfactory interneurons in males. In accordance with biochemical and behavioral data, the most potent are bombykal and two trienals, (E,E,E)- and (E,E,Z)-10,12,14-hexadecatrienal. We also conclude that the C15 dienal is selective for one of the trienal receptors on the antenna, but is much less potent than the natural trienal stimulant.