Micropathogen Community Analysis in Hyalomma rufipes via High-Throughput Sequencing of Small RNAs.
Micropathogen Community Analysis in Hyalomma rufipes via High-Throughput Sequencing of Small RNAs.
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通过小 RNA 高通量测序进行红斑透明疣微病原体群落分析
DOI：
10.3389/fcimb.2017.00374
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发表时间：
2017
影响因子：
5.7
通讯作者：
Liu GY
中科院分区：
文献类型：
--
作者：
Luo J;Liu MX;Ren QY;Chen Z;Tian ZC;Hao JW;Wu F;Liu XC;Luo JX;Yin H;Wang H;Liu GY
在将广泛的微病原体传播给包括人类在内的脊椎动物方面，扁虱是重要的媒介。由于硬蜱在疾病传播中的作用，鉴定和表征硬蜱种群的微病原菌谱变得越来越重要。本研究的目的是调查扇形透明线虫的病原菌。中国，利用Illumina HiSeq2000技术对甘肃省现场采集的皱纹夜蛾小RNA(SRNA)进行了深度测序。由此产生的sRNA文库数据显示，被调查的扁虱种群产生了与圣克罗伊河病毒(SCRV)序列同源的读取物。我们还观察到许多与微生物和/或致病菌株同源的读数，包括细菌、原生动物和真菌。作为分析的一部分，构建了系统发育树以显示所鉴定的同源序列之间的关系。这项研究提供了一个独一无二的机会，可以深入了解扁虱的微病原体。未来对节肢动物病媒的有效控制将需要了解携带感染性病原体的病媒的微生物组成。了解与微生物病原体谱系的流行和持久性相关的调节媒介传播的生态因素也是当务之急。这些相互作用可能会影响微病原体谱系的进化，特别是如果微病原体依赖载体或宿主进行传播的话。这项分析中使用的sRNA深度测序方法提供了一种直观的方法来调查蜱类和其他媒介物种中微病原体的流行情况。
Ticks are important vectors in the transmission of a broad range of micropathogens to vertebrates, including humans. Because of the role of ticks in disease transmission, identifying and characterizing the micropathogen profiles of tick populations have become increasingly important. The objective of this study was to survey the micropathogens of Hyalomma rufipes ticks. Illumina HiSeq2000 technology was utilized to perform deep sequencing of small RNAs (sRNAs) extracted from field-collected H. rufipes ticks in Gansu Province, China. The resultant sRNA library data revealed that the surveyed tick populations produced reads that were homologous to St. Croix River Virus (SCRV) sequences. We also observed many reads that were homologous to microbial and/or pathogenic isolates, including bacteria, protozoa, and fungi. As part of this analysis, a phylogenetic tree was constructed to display the relationships among the homologous sequences that were identified. The study offered a unique opportunity to gain insight into the micropathogens of H. rufipes ticks. The effective control of arthropod vectors in the future will require knowledge of the micropathogen composition of vectors harboring infectious agents. Understanding the ecological factors that regulate vector propagation in association with the prevalence and persistence of micropathogen lineages is also imperative. These interactions may affect the evolution of micropathogen lineages, especially if the micropathogens rely on the vector or host for dispersal. The sRNA deep-sequencing approach used in this analysis provides an intuitive method to survey micropathogen prevalence in ticks and other vector species.