Hippocampal AMPARs involve the central sensitization of rats with irritable bowel syndrome.
Hippocampal AMPARs involve the central sensitization of rats with irritable bowel syndrome.
复制标题
海马 AMPAR 涉及肠易激综合征大鼠的中枢敏化
DOI：
10.1002/brb3.650
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发表时间：
2017-03
影响因子：
3.1
通讯作者：
Lin C
中科院分区：
文献类型：
--
作者：
Chen A;Chen Y;Tang Y;Bao C;Cui Z;Xiao M;Lin C
研究了海马ampar在肠易激综合征（IBS）样大鼠中的作用，以阐明中枢致敏机制。采用新生儿产妇分离诱导IBS模型。通过双侧海马内注射CNQX（一种AMPAR抑制剂）后腹肌对结肠膨胀的反应来检测AMPAR对内脏超敏反应的影响。Western blot检测大鼠海马AMPARs （GluR1和GluR2）的表达。与对照组相比，IBS样大鼠表现出内脏过敏。双侧海马内注射CNQX可减轻IBS样大鼠的内脏疼痛。在CRD为40和60 mmHg的条件下，CNQX的最大作用时间为30 min，持续时间为90 min。与对照组相比，IBS样大鼠海马GluR2的表达显著增加（p < 0.05）。然而，大鼠海马GluR1水平无显著差异。与对照组相比，HFS诱导的海马LTP显著增强（p < 0.05）。对照组和IBS样大鼠的GluR2表达在录音结束60 min后显著升高（p < 0.05），而GluR1表达无显著升高。新生儿母亲分离可增强GluR2的表达，促进海马LTP的发生，长大后可形成内脏超敏反应。
The roles of hippocampal AMPARs were investigated in irritable bowel syndrome (IBS)‐like rats to clarify the central sensitization mechanisms. IBS model was induced by neonatal maternal separation. The effects of AMPARs on visceral hypersensitivity were examined by the responses of abdominal muscle to colorectal distension after the bilateral intrahippocampal injections of CNQX (an AMPAR inhibitor). The expressions of hippocampal AMPARs (GluR1 and GluR2) were determined by Western blot. The IBS‐like rats showed visceral hypersensitivity when compared with controls. Bilateral intrahippocampal injections of CNQX alleviated the visceral pain in IBS‐like rats. The maximal effect appeared at the time point of 30 min, and the duration lasted for 90 min after CNQX application, under 40 and 60 mmHg CRD. The expressions of hippocampal GluR2 significantly increased in IBS‐like rats when compared with controls (p < .05). However, the levels of hippocampal GluR1 had no significant differences in rats. Hippocampal LTP induced by HFS was significantly enhanced when compared with controls (p < .05). The expressions of GluR2 significantly increased in the control and IBS‐like rats after 60 min LTP of recordings (p < .05), but not GluR1. Neonatal maternal separation enhances the expression of GluR2 and facilitates the LTP in the hippocampus, which could lead to the formation of visceral hypersensitivity when grown up.