Role of bone marrow-derived mesenchymal stem cells in the prevention of hyperoxia-induced lung injury in newborn mice
Role of bone marrow-derived mesenchymal stem cells in the prevention of hyperoxia-induced lung injury in newborn mice
复制标题
骨髓间充质干细胞在预防新生小鼠高氧性肺损伤中的作用
DOI：
10.1042/cbi20110447
复制
发表时间：
2012-06-01
影响因子：
3.9
通讯作者：
Feng, Zhichun
中科院分区：
文献类型：
--
作者：
Zhang, Xiaoying;Wang, Hui;Feng, Zhichun
BPD（支气管肺发育不良）的主要特征是肺结构的持续异常和肺发育停滞，但治疗可以是姑息性的。虽然有希望，但BMSC（骨髓源性间充质干细胞）在肺部疾病治疗中的应用仍存在争议。我们已经评估了BMSC在体外和体内的治疗效果。在体外与损伤的肺组织共培养增加了BMSC的迁移潜力，并表达了AEC 2（II型肺泡上皮细胞）的特异性标志物SP-C（表面活性蛋白-C）。实验性BPD小鼠在出生后第7天腹腔注射BMSC后，发现BMSC可以归巢于损伤肺，表达SP-C，改善肺结构，减轻肺纤维化，提高BPD小鼠的存活率。这项工作支持的概念，即BMSC是通过生产可溶性因子的生物活性水平，调节炎症和纤维化的发病机制后高氧的治疗效益。
BPD (bronchopulmonary dysplasia) is predominantly characterized by persistent abnormalities in lung structure and arrested lung development, but therapy can be palliative. While promising, the use of BMSC (bone marrow-derived mesenchyrnal stem cell) in the treatment of lung diseases remains controversial. We have assessed the therapeutic effects of BMSC in vitro and in vivo. In vitro co-culturing with injured lung tissue increased the migration-potential of BMSC; and SP-C (surfactant protein-C), a specific marker of AEC2 (type II alveolar epithelial cells), was expressed. Following intraperitoneal injection of BMSC into experimental BPD mice on post-natal day 7, it was found that BMSC can home to the injured lung, express SP-C, improve pulmonary architecture, attenuate pulmonary fibrosis and increase the survival rate of BPD mice. This work supports the notion that BMSC are of therapeutic benefit through the production of soluble factors at bioactive levels that regulate the pathogenesis of inflammation and fibrosis following hyperoxia.