Correlation between axonal morphologies and synaptic input kinetics of interneurons from mouse visual cortex
Correlation between axonal morphologies and synaptic input kinetics of interneurons from mouse visual cortex
复制标题
DOI：
10.1093/cercor/bhj126
复制
发表时间：
2007-01-01
期刊：
影响因子：
3.7
通讯作者：
Yuste, Rafael
中科院分区：
文献类型：
--
作者：
Dumitriu, Daniella;Cossart, Rosa;Yuste, Rafael
新皮层中间神经元表现出巨大的形态和生理变异性，并被理想地定位于控制电路动力学，尽管它们的确切作用仍然知之甚少。为了更好地理解这种多样性，我们对从生长抑素、parvalburin和神经肽Y阳性神经元中表达绿色荧光蛋白的转基因小鼠分离的视觉皮层中间神经元的3种亚型进行了详细的解剖和生理表征。我们发现这三组中间神经元在树突和轴突形态上有系统的差异，并且它们所接收的自发兴奋性和抑制性突触电流的频率、幅度和动力学特征也不同。此外，我们检测到它们的突触输入动力学和轴突分支的定量方面之间的相关性。这表明不同的中间神经元类型可以引导不同的时间活动模式。我们的结果也证实了轴突形态对中间神经元分类的重要性。
Neocortical interneurons display great morphological and physiological variability and are ideally positioned to control circuit dynamics, although their exact role is still poorly understood. To better understand this diversity, we have performed a detailed anatomical and physiological characterization of 3 subtypes of visual cortex interneurons, isolated from transgenic mice which express green fluorescent protein in somatostatin, parvalburnin, and neuropeptide Y positive neurons. We find that these 3 groups of interneurons have systematic differences in dendritic and axonal morphologies and also characteristically differ in the frequencies, amplitude, and kinetics of the spontaneous excitatory and inhibitory synaptic currents they receive. Moreover, we detect a correlation between the kinetics of their synaptic inputs and quantitative aspects of their axonal arborizations. This suggests that different interneuron types could channel different temporal patterns of activity. Our results also confirm the importance of the axonal morphology to classify interneurons.