Analgesic effect of electroacupuncture on chronic neuropathic pain mediated by P2X3 receptors in rat dorsal root ganglion neurons
Analgesic effect of electroacupuncture on chronic neuropathic pain mediated by P2X3 receptors in rat dorsal root ganglion neurons
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DOI：
10.1016/j.neuint.2012.01.006
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发表时间：
2012-03-01
影响因子：
4.2
通讯作者：
Jiang, Song-he
中科院分区：
文献类型：
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作者：
Tu, Wen-zhan;Cheng, Rui-dong;Jiang, Song-he
腺苷三磷酸二钠（ATP）门控的P2 X受体，尤其是P2 X亚型（3），在神经病理性疼痛的疼痛信号传递中起着关键作用，ATP在疼痛信号的传递过程中起着重要作用，提示通过电针（EA）控制这些通路可能是治疗慢性神经病理性疼痛的有效方法。EA已被接受为通过针灸针将刺激电流施加到穴位来有效地管理慢性疼痛。为探讨电针对背根神经节（DRG）神经元P2 X（3）受体介导的神经病理性痛的影响，记录背根神经节（DRG）神经元的机械缩痛阈值（MWT）和热缩痛潜伏期（TWL），并采用免疫组织化学（IHC）和原位杂交（ISH）方法检测背根神经节（DRG）神经元P2 X（3）受体的表达。此外，通过实验性使用全细胞膜片钳记录，检测了由P2 X（3）受体激动剂即ATP和α，β-亚甲基-ATP（α，β-meATP）诱发的从慢性压迫性损伤（CCI）后的大鼠分离的DRG神经元中的电流。电针可增加CCI大鼠背根节神经元MWT和TWL值，降低P2 X（3）受体的表达。同时，电针治疗减弱ATP和α，β-meATP诱发的电流。电针可能通过降低CCI大鼠背根节神经元P2 X（3）受体的表达，抑制P2 X（3）受体的表达而产生明显的镇痛作用。电针对侧与同侧镇痛效果相似。(C)2012爱思唯尔有限公司保留所有权利。
Adenosine 5'-triphosphate disodium (ATP) gated P2X receptors, especially the subtype P2X(3), play a key role in transmission of pain signals in neuropathic pain, ATP has been documented to play a significant role in the progression of pain signals, suggesting that control of these pathways through electroacupuncture (EA) is potentially an effective treatment for chronic neuropathic pain. EA has been accepted to effectively manage chronic pain by applying the stimulating current to acupoints through acupuncture needles. To determine the significance of EA on neuropathic pain mediated by P2X(3) receptors in the dorsal root ganglion (DRG) neurons, mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were recorded, and the expression of P2X(3) receptors in the DRG neurons was assessed by immunohistochemistry (IHC) and in situ hybridization (ISH). In addition, the currents which were evoked in DRG neurons isolated from rats following chronic constriction injury (CCI) by the P2X(3) receptors agonists i.e. ATP and alpha,beta-methylen-ATP (alpha,beta-meATP) were examined through the experimental use of whole cell patch clamp recording. The present study demonstrates that EA treatment can increase the MWT and TWL values and decrease the expression of P2X(3) receptors in DRG neurons in CCI rats. Simultaneously, EA treatment attenuates the ATP and alpha,beta-meATP evoked currents. EA may be expected to induce an apparent induce analgesic effect by decreasing expression and inhibiting P2X(3) receptors in DRG neurons of CCI rats. There is a similar effect on analgesic effect between rats with contralateral EA and those with ipsilateral EA. (C) 2012 Elsevier Ltd. All rights reserved.