Evaluation of UW solution for preservation of small intestinal transplants in the rat.
Evaluation of UW solution for preservation of small intestinal transplants in the rat.
复制标题
UW 溶液保存大鼠小肠移植物的评价。
DOI：
10.1097/00007890-199206000-00006
复制
发表时间：
1992
期刊：
影响因子：
6.2
通讯作者：
Frank M. Guttman
中科院分区：
文献类型：
--
作者：
Tomoaki Taguchi;Edith Zorychta;Frank M. Guttman
在大鼠小肠移植模型上，对单纯低温保存进行了评价。刘易斯大鼠立即（SI）或在Euro-Collins（SPE 24）中保存24小时、在EC（SPE 48）中保存48小时、在威斯康星州大学溶液（SPW 24）中保存24小时或在UW（SPW 48）中保存48小时后接受空肠的同系异位移植物。S1、SPE 24、SPE 48、SPW 24和SPW 48的存活率分别为100%、78%、0%、100%和33%。移植后8 - 12天，在体外评价移植物和天然肠的生理和药理学特性。所有标本的平滑肌收缩胆碱能激动剂，苯肾上腺素，P物质，并放松异丙肾上腺素。SI、SPE 24、SPW 24和SPW 48的兴奋性神经支配分别为100%、100%、100%和67%，而抑制性神经支配分别为50%、29%、60%和0%。因此，在所有组中均保留了平滑肌功能，但神经活性因某些储存条件而受损。SPW 24保存效果最好，其生理反应与SI相似。因此，大鼠空肠可以在移植前使用UW溶液中的简单冷藏在良好的条件下保存长达24小时。
Simple cold preservation was evaluated in the rat model of small intestinal transplantation. Lewis rats received a syngeneic heterotopic graft of the jejunum either immediately (SI) or after preservation for 24 hr in Euro-Collins (SPE24), for 48 hr in EC (SPE48), for 24 hr in University Wisconsin solution (SPW24), or for 48 hr in UW (SPW48). The survival rates of SI, SPE24, SPE48, SPW24, and SPW48 were 100%, 78%, 0%, 100%, and 33%, respectively. Physiologic and pharmacologic properties of the grafts and native intestine were evaluated in vitro between 8 and 12 days after transplantation. Smooth muscle in all specimens contracted in response to cholinergic agonists, phenylephrine, and substance P, and was relaxed by isoproterenol. Excitatory innervation was present in 100%, 100%, 100%, and 67% of SI, SPE24, SPW24, and SPW48, respectively, while inhibitory innervation in each group was 50%, 29%, 60%, and 0%. Thus, smooth muscle function was preserved in all groups, but neural activity was impaired by some of the storage conditions. Preservation was best in SPW24, which had physiologic responses similar to those of SI. The rat jejunum can, therefore, be preserved in good condition for up to 24 hr before transplantation using simple cold storage in UW solution.