Topical anti-inflammatory activity of Eugenia brasiliensis Lam. (Myrtaceae) leaves
Topical anti-inflammatory activity of Eugenia brasiliensis Lam. (Myrtaceae) leaves
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DOI：
10.1211/jpp.60.4.0011
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发表时间：
2008-04-01
影响因子：
3.3
通讯作者：
Otuki, Michel Fleith
中科院分区：
文献类型：
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作者：
Pietrovski, Evelise Fernandes;Magina, Michele Debiasi Alberton;Otuki, Michel Fleith
巴西丁香是一种产自巴西南部的植物，用于治疗风湿病的流行药物。这项研究报告说，局部应用水乙醇提取物，部分和分离的化合物从巴西藤本植物引起的耳肿胀的反应，局部使用巴豆油对小鼠的耳朵。水提物及其提取物(正己烷、乙酸乙酯和二氯甲烷)的ID50分别为0.17、0.29、0.13和0.14 mg/耳，抑制率分别为79+/-7%、87+/-6%、88+/-5%和96+/-2%。分离的酚类化合物(槲皮素、儿茶素和没食子儿茶素)也能有效地抑制水肿胀，抑制率分别为61.5%、66.2%和37+/-9%。此外，提取物和分离出的化合物均能抑制中性粒细胞内流(抑制率分别为85+/-6%、81+/-5%、73+/-6%和76+/-6%)。耳部组织的组织学分析清楚地证实，巴豆油提取物和化合物能抑制巴豆油对小鼠耳部皮肤的中性粒细胞流入。此外，水提物对花生四烯酸所致小鼠耳肿胀也有明显的抑制作用(ID50为1.94 mg/耳，抑制率为60+/-7%)。因此，这些结果一致支持巴西棘球藻具有局部抗炎活性的观点。
Eugenia brasiliensis Lam., a plant from the south of Brazil, is used in the popular medicine for rheumatism treatment. This study reports that topical application of hydroalcoholic extract, fractions and isolated compounds from E. brasiliensis caused an inhibition of ear oedema in response to topical application of croton oil on the mouse ear. For oedema inhibition, the estimated ID50 values (dose reducing the inflammatory response by 50% relative to the control value) for hydroalcoholic extract and fractions (hexane, ethyl acetate and dichloromethane) were 0.17, 0.29, 0.13 and 0.14 mg/ear, respectively, with inhibition of 79 +/- 7%, 87 +/- 6%, 88 +/- 5% and 96 +/- 2%, respectively. Isolated phenolic compounds (quercetin, catechin and gallocatechin) were also effective in inhibiting the oedema (inhibition of 61 5%, 66 2% and 37 +/- 9%, respectively). Moreover, both extract and isolated compounds caused inhibition of polymorphonuclear cells influx (inhibition of 85 +/- 6%, 81 +/- 5%, 73 +/- 6% and 76 +/- 6%, respectively). The histological analysis of the ear tissue clearly confirmed that the extract and compounds of E. brasiliensis inhibited the influx of polymorphonuclear cells to mouse ear skin after application of croton oil. Furthermore, hydroalcoholic extract was also effective in inhibiting the arachiclonic acid-mediated mouse ear oedema (ID50 value was 1.94 mg/ear and inhibition of 60 +/- 7%). Therefore, these results consistently support the notion that E. brasiliensis possesses topical anti-inflammatory activity.